Epithelial polarity proteins regulate Drosophila tracheal tube size in parallel to the luminal matrix pathway.
Epithelial polarity proteins regulate Drosophila tracheal tube size in parallel to the luminal matrix pathway.
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DOI：
10.1016/j.cub.2009.11.017
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发表时间：
2010-01-12
期刊：
影响因子：
--
通讯作者：
Tepass U
中科院分区：
文献类型：
--
作者：
Laprise P;Paul SM;Boulanger J;Robbins RM;Beitel GJ;Tepass U
上皮管大小的调节对器官功能至关重要。然而，管尺寸控制的机制仍然知之甚少。在果蝇气管中，管的尺寸由管腔细胞外基质（ECM）调节。ECM组织需要依赖于基底外侧分隔连接（SJ）的蛋白蠕虫状（Verm）的顶端（管腔）分泌。在这里，我们表明，顶端和基底侧上皮极性蛋白相互作用，以控制气管大小独立的蠕虫途径。蒙古包（yrt）和scribble（scribb）的突变，编码SJ相关极性蛋白，导致气管扩张，但不破坏蠕虫分泌。减少活性的顶端极性蛋白Crumbs（Crb）抑制的长度缺陷的YRT，但不scrib突变体，这表明YRT的行为负调控Crb。相反，Crb过表达增加气管导管尺寸。减少crb剂量也可以挽救由编码sj相关极性蛋白的cora基因突变引起的气管大小缺陷。此外，crb突变抑制cora长度缺陷而不恢复Verm分泌。总之，这些数据表明，Yrt，Cora，Crb和Scrib独立于Verm途径运作。我们的数据支持一个模型，其中Cora和Yrt通过Crb调节上皮管的大小。
Regulation of epithelial tube size is critical for organ function. However, the mechanisms of tube-size control remain poorly understood. In the Drosophila trachea, tube dimensions are regulated by a luminal extracellular matrix (ECM). ECM organization requires apical (luminal) secretion of the protein Vermiform (Verm), which depends on the basolateral septate junction (SJ). Here, we show that apical and basolateral epithelial polarity proteins interact to control tracheal tube-size independently of the Verm pathway. Mutations in yurt (yrt) and scribble (scrib), which encode SJ-associated polarity proteins, cause an expansion of tracheal tubes, but do not disrupt Verm secretion. Reducing activity of the apical polarity protein Crumbs (Crb) suppresses the length defects in yrt but not scrib mutants, suggesting that Yrt acts by negatively regulating Crb. Conversely, Crb overexpression increases tracheal tube dimensions. Reducing crb dosage also rescues tracheal size defects caused by mutations in coracle (cora), which encodes a SJ-associated polarity protein. In addition, crb mutations suppress cora length defects without restoring Verm secretion. Together, these data indicate that Yrt, Cora, Crb and Scrib operate independently of the Verm pathway. Our data support a model in which Cora and Yrt act through Crb to regulate epithelial tube size.
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影响因子：
10.5
作者：
Harris PC;Torres VE
通讯作者：
Torres VE
影响因子：
11.8
作者：
Behr, M;Riedel, D;Schuh, R
通讯作者：
Schuh, R
影响因子：
2.7
作者：
Kerman, Bilal E.;Cheshire, Alan M.;Andrew, Deborah J.
通讯作者：
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果蝇致命（2）巨型幼虫抑制蛋白是细胞骨架的组成部分。
DOI：
10.1083/jcb.127.5.1345
发表时间：
1994-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Strand D;Raska I;Mechler BM
通讯作者：
Mechler BM
影响因子：
3.3
作者：
Lamb, RS;Ward, RE;Fehon, RG
通讯作者：
Fehon, RG