Effects of icariin on the fracture healing in young and old rats and its mechanism.
Effects of icariin on the fracture healing in young and old rats and its mechanism.
复制标题
淫羊藿苷对幼年和老年大鼠骨折愈合的影响及其机制
DOI：
10.1080/13880209.2021.1972121
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发表时间：
2021-12
影响因子：
3.8
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Zhang X;Chen Y;Zhang C;Zhang X;Xia T;Han J;Song S;Xu C;Chen F
摘要内容淫羊藿苷因其广泛的药理作用而受到越来越多的关注。目的探讨淫羊藿苷对青年和老年大鼠骨折愈合的促进作用及其机制。材料与方法建立Wistar大鼠胫骨骨折模型。将大鼠分为模型组、L-淫羊藿苷（50 mg/kg淫羊藿苷）组、M-淫羊藿苷（100 mg/kg淫羊藿苷）组和H-淫羊藿苷（200 mg/kg淫羊藿苷）组，分别灌胃给予淫羊藿苷10 d和20 d。另外，将分离培养的青年和老年大鼠骨髓间充质干细胞（rBMSCs）分别与5%和20%淫羊藿苷含药血清共同培养，测定细胞活力和碱性磷酸酶（ALP）活性。结果淫羊藿苷诱导成骨细胞Runx 2、Osterix、BMP-2、p-Smad 5表达及骨钙素分泌（青年大鼠：模型：2.50 ± 0.71; L-淫羊藿苷：10.10 ± 1.55; M-淫羊藿苷：24.95 ± 2.19; H-淫羊藿苷：36.80 ± 2.26;老年大鼠：模型：1.55 ± 0.49; L-淫羊藿苷：6.55 ± 0.50; M-淫羊藿苷：15.00 ± 0.85; H-淫羊藿苷：20.50 ± 2.27），并以剂量依赖方式增加骨形成标志物（OC，BAP，NTX-1和CTX-1）的水平。体外实验中，淫羊藿苷处理可促进rBMSC的存活，增加ALP活性和BMP-2/Smad 5/Runx 2通路蛋白的表达。讨论和结论淫羊藿苷可能通过激活BMP-2/Smad 5/Runx 2通路促进相对年轻和老年大鼠的骨折愈合。对淫羊藿苷促进骨折愈合机制的研究可为淫羊藿苷的临床应用和推广提供理论依据。
Abstract Context Icariin has attracted increasing attention because of its wide variety of pharmacological effects. Objective This study investigates whether icariin could promote fracture healing in young and old rats and its mechanisms. Materials and methods A Wistar rat model for the tibia fracture in relatively young and old rats, respectively, was established. The rats were divided into four groups: model group, L-icariin (50 mg/kg icariin), M-icariin (100 mg/kg icariin) and H-icariin (200 mg/kg icariin), and intragastric administration of icariin was performed for 10 days or 20 days. In addition, isolated and cultured rat bone mesenchymal stem cells (rBMSCs) from young and old rats were cultured with 5% and 20% of icariin-containing serum, respectively, then cell viability and alkaline phosphatase (ALP) activity were measured. Results Icariin administration induced the expression of Runx2, Osterix, BMP-2, p-Smad5 and osteocalcin secretion (young rats: model: 2.50 ± 0.71; L-icariin: 10.10 ± 1.55; M-icariin: 24.95 ± 2.19; H-icariin: 36.80 ± 2.26; old rats: model: 1.55 ± 0.49; L-icariin:6.55 ± 0.50; M-icariin: 15.00 ± 0.85; H-icariin:20.50 ± 2.27) at the fracture site, and increased the levels of bone formation markers (OC, BAP, NTX-1 and CTX-1) in a dose-dependent manner. In vitro, icariin treatment promoted rBMSC viability, increased ALP activity and the expression of BMP-2/Smad5/Runx2 pathway proteins. Discussion and conclusions Icariin may accelerate fracture healing by activating the BMP-2/Smad5/Runx2 pathway in relatively young and old rats. The research on the mechanism of icariin to promote fracture healing can provide a theoretical basis for the clinical application and promotion of icariin.
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影响因子：
9.2
作者：
Dai J;Li Y;Zhou H;Chen J;Chen M;Xiao Z
通讯作者：
Xiao Z
影响因子：
5.2
作者：
Bailey S;Karsenty G;Gundberg C;Vashishth D
通讯作者：
Vashishth D
影响因子：
3.6
作者：
Aydin, Ali;Halici, Zekai;Kovaci, Halim
通讯作者：
Kovaci, Halim
DOI：
10.3969/j.issn.1003-0034.2017.08.013
发表时间：
2017-08-25
影响因子：
--
作者：
Shi, Xiao-Lin;Liang, Bo-Cheng;Li, Chun-Wen
通讯作者：
Li, Chun-Wen
影响因子：
2.3
作者：
Wang, Ching-Jen;Liu, Hao-Chen;Fu, Te-Hu
通讯作者：
Fu, Te-Hu