Integrated Systems Analysis of Mixed Neuroglial Cultures Proteome Post Oxycodone Exposure.
Integrated Systems Analysis of Mixed Neuroglial Cultures Proteome Post Oxycodone Exposure.
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DOI：
10.3390/ijms22126421
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发表时间：
2021-06-15
影响因子：
5.6
通讯作者：
Pendyala G
中科院分区：
文献类型：
--
作者：
Guda RS;Odegaard KE;Tan C;Schaal VL;Yelamanchili SV;Pendyala G
阿片类药物滥用已成为影响全球数百万人的重大公共卫生危机。处方阿片类药物的广泛滥用和非法阿片类药物供应的急剧增加造成了所谓的阿片类药物流行。孕妇是一个特别脆弱的群体，因为她们被开出了吗啡、丁丙诺啡和美沙酮等阿片类药物的处方，所有这些都被证明可以穿过胎盘，并可能影响发育中的胎儿。关于羟考酮(OXY)对突触改变的影响，目前的信息有限。为了填补这一知识空白，我们采用了一种集成的系统方法来确定OXY处理24小时后对混合神经胶质细胞培养产生影响的蛋白质组特征和途径。利用独创性途径分析(IPA)将差异表达的蛋白质映射到全球规范途径上，识别丰富的与ePhin信号、信号素信号、突触长期抑制、内源性大麻素信号和阿片信号相关的通路。ClueGO的进一步分析发现，差异表达的蛋白质功能的主要类别与GDP结合有关。由于阿片受体是G蛋白偶联受体(GPCRs)，这些数据表明，氧暴露扰乱了与突触功能相关的关键通路。
Opioid abuse has become a major public health crisis that affects millions of individuals across the globe. This widespread abuse of prescription opioids and dramatic increase in the availability of illicit opioids have created what is known as the opioid epidemic. Pregnant women are a particularly vulnerable group since they are prescribed for opioids such as morphine, buprenorphine, and methadone, all of which have been shown to cross the placenta and potentially impact the developing fetus. Limited information exists regarding the effect of oxycodone (oxy) on synaptic alterations. To fill this knowledge gap, we employed an integrated system approach to identify proteomic signatures and pathways impacted on mixed neuroglial cultures treated with oxy for 24 h. Differentially expressed proteins were mapped onto global canonical pathways using ingenuity pathway analysis (IPA), identifying enriched pathways associated with ephrin signaling, semaphorin signaling, synaptic long-term depression, endocannabinoid signaling, and opioid signaling. Further analysis by ClueGO identified that the dominant category of differentially expressed protein functions was associated with GDP binding. Since opioid receptors are G-protein coupled receptors (GPCRs), these data indicate that oxy exposure perturbs key pathways associated with synaptic function.
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影响因子：
2.2
作者：
Naghshvarian M;Zarrindast MR;Mehr SE;Ommati MM;Sajjadi SF
通讯作者：
Sajjadi SF
影响因子：
5.9
作者：
Chand S;Jo A;Vellichirammal NN;Gowen A;Guda C;Schaal V;Odegaard K;Lee H;Pendyala G;Yelamanchili SV
通讯作者：
Yelamanchili SV
影响因子：
4.6
作者：
Liu R;Dang W;Du Y;Zhou Q;Jiao K;Liu Z
通讯作者：
Liu Z
影响因子：
5.5
作者：
Odegaard KE;Schaal VL;Clark AR;Koul S;Sankarasubramanian J;Xia Z;Mellon M;Uberti M;Liu Y;Stothert A;Van Hook M;Wang H;Guda C;Lisco SJ;Pendyala G;Yelamanchili SV
通讯作者：
Yelamanchili SV
GNAI1 和 GNAI3 通过阻断 IL6 信号传导和下调 GNAI2 的表达来减少小鼠结肠炎相关的肿瘤发生
影响因子：
29.4
作者：
Li，Zhi-Wei;Sun，Beicheng;Chu，Wen-Ming
通讯作者：
Chu，Wen-Ming