Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest.
Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest.
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DOI：
10.1038/srep34376
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发表时间：
2016-09-29
影响因子：
4.6
通讯作者：
Jena KK
中科院分区：
文献类型：
--
作者：
Ji H;Kim SR;Kim YH;Suh JP;Park HM;Sreenivasulu N;Misra G;Kim SM;Hechanova SL;Kim H;Lee GS;Yoon UH;Kim TH;Lim H;Suh SC;Yang J;An G;Jena KK
褐飞虱是一种为害水稻的韧皮部吸汁害虫，严重危害水稻产量。我们克隆了来自野生稻物种澳大利亚的抗BPH渗入系的BPH 18基因。图位克隆和互补试验表明，BPH 18编码CC-NBS-NBS-LRR蛋白。与典型的NBS-LRR蛋白不同，BPH 18具有两个NBS结构域。BPH 18启动子：：GUS转基因植株在叶鞘的维管束中表现出强烈的GUS表达，特别是在褐飞虱攻击的韧皮部细胞中。BPH 18蛋白广泛定位于细胞内膜，包括内质网、高尔基体、高尔基体网络和前液泡区室，表明BPH 18可能识别韧皮部细胞内膜上的BPH入侵。近等基因系NIL-BPH 18和NIL-BPH 26的全基因组测序结果表明，BPH 18与BPH 26位于同一位点。然而，这两个基因有显着的序列差异和独立的近等基因系表现出不同的抗褐飞虱与植物防御相关基因的表达模式，表明BPH 18和BPH 26是功能不同的等位基因。这些结果有助于阐明水稻抗褐飞虱的分子机制，并为快速选育抗褐飞虱水稻品种提供新的等位基因。
Brown planthopper (BPH) is a phloem sap-sucking insect pest of rice which causes severe yield loss. We cloned the BPH18 gene from the BPH-resistant introgression line derived from the wild rice species Oryza australiensis. Map-based cloning and complementation test revealed that the BPH18 encodes CC-NBS-NBS-LRR protein. BPH18 has two NBS domains, unlike the typical NBS-LRR proteins. The BPH18 promoter::GUS transgenic plants exhibited strong GUS expression in the vascular bundles of the leaf sheath, especially in phloem cells where the BPH attacks. The BPH18 proteins were widely localized to the endo-membranes in a cell, including the endoplasmic reticulum, Golgi apparatus, trans-Golgi network, and prevacuolar compartments, suggesting that BPH18 may recognize the BPH invasion at endo-membranes in phloem cells. Whole genome sequencing of the near-isogenic lines (NILs), NIL-BPH18 and NIL-BPH26, revealed that BPH18 located at the same locus of BPH26. However, these two genes have remarkable sequence differences and the independent NILs showed differential BPH resistance with different expression patterns of plant defense-related genes, indicating that BPH18 and BPH26 are functionally different alleles. These findings would facilitate elucidation of the molecular mechanism of BPH resistance and the identified novel alleles to fast track breeding BPH resistant rice cultivars.
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影响因子：
11.6
作者：
Liu, Tzu-Yin;Huang, Teng-Kuei;Chiou, Tzyy-Jen
通讯作者：
Chiou, Tzyy-Jen
影响因子：
11.4
作者：
Jia, Y;McAdams, SA;Valent, B
通讯作者：
Valent, B
BAC 和 RNA 测序揭示重组冷点中的褐飞虱抗性基因 BPH15 介导独特的防御机制
影响因子：
4.4
作者：
Lv W;Du B;Shangguan X;Zhao Y;Pan Y;Zhu L;He Y;He G
通讯作者：
He G
影响因子：
7.2
作者：
Hayashi, Nagao;Inoue, Haruhiko;Takatsuji, Hiroshi
通讯作者：
Takatsuji, Hiroshi
影响因子：
7.4
作者：
Klingler, J;Creasy, R;Singh, KB
通讯作者：
Singh, KB