Dawn- and dusk-phased circadian transcription rhythms coordinate anabolic and catabolic functions in Neurospora.
Dawn- and dusk-phased circadian transcription rhythms coordinate anabolic and catabolic functions in Neurospora.
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DOI：
10.1186/s12915-015-0126-4
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发表时间：
2015-02-24
期刊：
影响因子：
5.4
通讯作者：
Brunner M
中科院分区：
文献类型：
--
作者：
Sancar C;Sancar G;Ha N;Cesbron F;Brunner M
生物钟控制大量基因的节律表达，与24小时昼夜循环协调。产生昼夜节律的机制，其幅度和昼夜相位依赖于极其复杂的转录网络。此外，转录后机制在RNA丰度产生节律中的作用尚不清楚。在这里，我们分析了时钟控制的粗糙脉孢菌的转录组连同延长RNA聚合酶II的时间配置文件。我们的数据表明，转录有助于脉孢菌中绝大多数时钟控制基因（ccgs）的节律表达。ccg聚集在两个主要的簇中，在黎明或黄昏时达到转录和表达水平的峰值。黎明期基因主要参与合成代谢过程中的分解代谢和黄昏期基因，表明脉孢菌生理学的时钟控制时间分离。其表达强烈依赖于核心昼夜节律激活剂WCC的基因主要落入黎明阶段的集群，而由葡萄糖依赖性阻遏物CSP 1调节的节律基因主要落入黄昏阶段的集群。令人惊讶的是，在没有CSP 1的情况下，节律性转录物的数量增加了约两倍，表明许多基因的节律性表达被CSP 1的活性减弱。这些数据表明脉孢菌中绝大多数的转录节律是由黎明和黄昏特异性转录产生的。我们的观察结果表明，大量的可塑性的昼夜节律转录组的节奏基因的数量以及振幅和相位的表达节奏，并强调了一个重要的作用，在时间组织的代谢和生理的生物钟。本文的在线版本（doi：10.1186/s12915-015-0126-4）包含补充材料，可供授权用户使用。
Circadian clocks control rhythmic expression of a large number of genes in coordination with the 24 hour day-night cycle. The mechanisms generating circadian rhythms, their amplitude and circadian phase are dependent on a transcriptional network of immense complexity. Moreover, the contribution of post-transcriptional mechanisms in generating rhythms in RNA abundance is not known. Here, we analyzed the clock-controlled transcriptome of Neurospora crassa together with temporal profiles of elongating RNA polymerase II. Our data indicate that transcription contributes to the rhythmic expression of the vast majority of clock-controlled genes (ccgs) in Neurospora. The ccgs accumulate in two main clusters with peak transcription and expression levels either at dawn or dusk. Dawn-phased genes are predominantly involved in catabolic and dusk-phased genes in anabolic processes, indicating a clock-controlled temporal separation of the physiology of Neurospora. Genes whose expression is strongly dependent on the core circadian activator WCC fall mainly into the dawn-phased cluster while rhythmic genes regulated by the glucose-dependent repressor CSP1 fall predominantly into the dusk-phased cluster. Surprisingly, the number of rhythmic transcripts increases about twofold in the absence of CSP1, indicating that rhythmic expression of many genes is attenuated by the activity of CSP1. The data indicate that the vast majority of transcript rhythms in Neurospora are generated by dawn and dusk specific transcription. Our observations suggest a substantial plasticity of the circadian transcriptome with respect to the number of rhythmic genes as well as amplitude and phase of the expression rhythms and emphasize a major role of the circadian clock in the temporal organization of metabolism and physiology. The online version of this article (doi:10.1186/s12915-015-0126-4) contains supplementary material, which is available to authorized users.
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影响因子：
9.8
作者：
Jouffe C;Cretenet G;Symul L;Martin E;Atger F;Naef F;Gachon F
通讯作者：
Gachon F
影响因子：
11.3
作者：
Baker CL;Loros JJ;Dunlap JC
通讯作者：
Dunlap JC
影响因子：
29
作者：
Hatori M;Vollmers C;Zarrinpar A;DiTacchio L;Bushong EA;Gill S;Leblanc M;Chaix A;Joens M;Fitzpatrick JA;Ellisman MH;Panda S
通讯作者：
Panda S
昼夜节律的代谢和能量学的融合。
DOI：
10.1126/science.1195027
发表时间：
2010-12-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bass J;Takahashi JS
通讯作者：
Takahashi JS
影响因子：
8
作者：
Halbeisen, R. E.;Galgano, A.;Scherrer, T.;Gerber, A. P.
通讯作者：
Gerber, A. P.