Establishment and Characterization of Stable Zein/Glycosylated Lactoferrin Nanoparticles to Enhance the Storage Stability and in vitro Bioaccessibility of 7,8-Dihydroxyflavone.
Establishment and Characterization of Stable Zein/Glycosylated Lactoferrin Nanoparticles to Enhance the Storage Stability and in vitro Bioaccessibility of 7,8-Dihydroxyflavone.
复制标题
DOI：
10.3389/fnut.2021.806623
复制
发表时间：
2021
影响因子：
5
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Chen Y;Gao X;Liu S;Cai Q;Wu L;Sun Y;Xia G;Wang Y
本研究以乳铁蛋白（LF）为原料，通过Maillard反应，以分子量为10、40和70 kDa的右旋糖酐（LF 10 K、LF 40 K和LF 70 K）为稳定剂，对LF进行糖基化修饰，制备了玉米醇溶蛋白/糖基化LF纳米粒，并对7，8-二羟基黄酮（7，8-DHF）进行了包封。通过疏水作用和氢键作用成功制备了3种低浊度（<0.220）和多分散指数（PDI）（<0.230）的玉米醇溶蛋白/糖基化LF纳米粒（79.27-87.24 nm）。与玉米醇溶蛋白/LF纳米颗粒相比，玉米醇溶蛋白/糖基化LF纳米颗粒进一步增加了在低pH条件下对离子强度（0-500 mM NaCl）的稳定性。玉米醇溶蛋白/糖基化LF纳米颗粒呈纳米级球形，糖基化LF改变了玉米醇溶蛋白纳米颗粒的表面形貌。此外，包封的7，8-DHF在玉米醇溶蛋白/糖基化LF纳米颗粒内表现出无定形状态。最重要的是，玉米醇溶蛋白/糖基化LF纳米粒具有良好的保水性、较高的包封率（98.50%以上）、良好的储存稳定性以及对7，8-DHF，特别是LF 40 K的生物可及性。以上研究为玉米醇溶蛋白载体的应用提供了理论参考。
In this work, the lactoferrin (LF) was glycosylated by dextran (molecular weight 10, 40, and 70 kDa, LF 10K, LF 40K, and LF 70K) via Maillard reaction as a stabilizer to establish zein/glycosylated LF nanoparticles and encapsulate 7,8-dihydroxyflavone (7,8-DHF). Three zein/glycosylated LF nanoparticles (79.27–87.24 nm) with low turbidity (<0.220) and polydispersity index (PDI) (<0.230) were successfully established by hydrophobic interactions and hydrogen bonding. Compared with zein/LF nanoparticles, zein/glycosylated LF nanoparticles further increased stability to ionic strength (0–500 mM NaCl) at low pH conditions. Zein/glycosylated LF nanoparticles had nanoscale spherical shape and glycosylated LF changed surface morphology of zein nanoparticles. Besides, encapsulated 7,8-DHF exhibited an amorphous state inside zein/glycosylated LF nanoparticles. Most importantly, zein/glycosylated LF nanoparticles had good water redispersibility, high encapsulation efficiency (above 98.50%), favorable storage stability, and bioaccessibility for 7,8-DHF, particularly LF 40K. Collectively, the above research provides a theoretical reference for the application of zein-based delivery systems.
登录
查看更多内容
DOI：
10.1111/j.1601-183x.2012.00801.x
发表时间：
2012-07
期刊：
Genes, brain, and behavior
影响因子：
--
作者：
Andero R;Ressler KJ
通讯作者：
Ressler KJ
影响因子：
5.6
作者：
Chen, Yufeng;Xia, Guobin;Zhang, Ying
通讯作者：
Zhang, Ying
影响因子：
5
作者：
Aytan N;Choi JK;Carreras I;Crabtree L;Nguyen B;Lehar M;Blusztajn JK;Jenkins BG;Dedeoglu A
通讯作者：
Dedeoglu A
影响因子：
8.8
作者：
Hu, Kun;Huang, Xiaoxia;McClements, David Julian
通讯作者：
McClements, David Julian
影响因子：
6.1
作者：
Davidov-Pardo, Gabriel;Joye, Iris J.;McClements, David Julian
通讯作者：
McClements, David Julian