Application of Tandem Two-Dimensional Mass Spectrometry for Top-Down Deep Sequencing of Calmodulin.
Application of Tandem Two-Dimensional Mass Spectrometry for Top-Down Deep Sequencing of Calmodulin.
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DOI：
10.1007/s13361-018-1978-y
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发表时间：
2018-08
影响因子：
3.2
通讯作者：
O'Connor PB
中科院分区：
文献类型：
--
作者：
Floris F;Chiron L;Lynch AM;Barrow MP;Delsuc MA;O'Connor PB
二维质谱法（2DMS）涉及通过系统地调制前体离子通过碎裂区来关联混合物中的所有分析物的前体离子和碎片离子，从而同时采集混合物中的所有分析物的碎裂模式。串联二维质谱（MS/2DMS）结合了傅里叶变换离子回旋共振（FT-ICR）MS/MS的超高准确度和2DMS的同时数据独立碎片化，以实现整个蛋白质的广泛残基间碎片化。2DMS是近年来发展起来的一种自上而下的蛋白质组学（TDP）技术，并应用于钙调素（CaM）的分析，报道了红外多光子解离（IRMPD）作为片段化技术的切割覆盖率约为23%。这项工作的目标是扩大使用MS/2DMS的自上而下的蛋白质分析的实用性，以便将自上而下的蛋白质组学中的切割覆盖范围进一步扩展到蛋白质的内部区域。在这种情况下，使用MS/2DMS，CaM的切割覆盖率从约23%增加到约42%。二维质谱，当应用于主要碎片离子从源，允许深度测序的蛋白质钙调素。本文的在线版本（10.1007/s13361-018-1978-y）包含补充材料，可供授权用户使用。
Two-dimensional mass spectrometry (2DMS) involves simultaneous acquisition of the fragmentation patterns of all the analytes in a mixture by correlating their precursor and fragment ions by modulating precursor ions systematically through a fragmentation zone. Tandem two-dimensional mass spectrometry (MS/2DMS) unites the ultra-high accuracy of Fourier transform ion cyclotron resonance (FT-ICR) MS/MS and the simultaneous data-independent fragmentation of 2DMS to achieve extensive inter-residue fragmentation of entire proteins. 2DMS was recently developed for top-down proteomics (TDP), and applied to the analysis of calmodulin (CaM), reporting a cleavage coverage of about ~23% using infrared multiphoton dissociation (IRMPD) as fragmentation technique. The goal of this work is to expand the utility of top-down protein analysis using MS/2DMS in order to extend the cleavage coverage in top-down proteomics further into the interior regions of the protein. In this case, using MS/2DMS, the cleavage coverage of CaM increased from ~23% to ~42%. Two-dimensional mass spectrometry, when applied to primary fragment ions from the source, allows deep-sequencing of the protein calmodulin. The online version of this article (10.1007/s13361-018-1978-y) contains supplementary material, which is available to authorized users.
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影响因子：
64.5
作者：
Cox, Juergen;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
7.4
作者：
Bray, Fabrice;Bouclon, Julien;Rolando, Christian
通讯作者：
Rolando, Christian
影响因子：
4.3
作者：
van Agthoven, Maria A.;Delsuc, Marc-Andre;Rolando, Christian
通讯作者：
Rolando, Christian
影响因子：
1.8
作者：
van Agthoven, Maria A.;Delsuc, Marc-Andre;Rolando, Christian
通讯作者：
Rolando, Christian
影响因子：
4.2
作者：
Simon, H. J.;van Agthoven, M. A.;O'Connor, P. B.
通讯作者：
O'Connor, P. B.