Structure and function of the lanthanide-dependent methanol dehydrogenase XoxF from the methanotroph Methylomicrobium buryatense 5GB1C.
Structure and function of the lanthanide-dependent methanol dehydrogenase XoxF from the methanotroph Methylomicrobium buryatense 5GB1C.
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DOI：
10.1007/s00775-018-1604-2
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发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Rosenzweig AC
中科院分区：
文献类型：
--
作者：
Deng YW;Ro SY;Rosenzweig AC
在使用一碳（C1）化合物作为碳源的甲基营养细菌中，甲醇被吡咯喹啉醌（PQQ）依赖性甲醇脱氢酶（MDH）氧化。甲基营养型基因组通常编码两种不同的MDH，MxaF和XoxF。MxaF是一种研究充分的钙依赖性异源四聚体酶，而XoxF是镧系元素依赖性同源二聚体。最近的研究表明，XoxF可能是在许多环境中的功能MDH。在甲烷营养菌中，利用甲烷的甲基营养菌，已经检测到颗粒甲烷单加氧酶（pMMO）和MxaF之间的相互作用。为了研究pMMO和XoxF之间相互作用的可能性，从甲烷氧化菌Buryatense甲基微菌5GB 1C（5G-XoxF）中分离XoxF。纯化的5G-XoxF的比活性为0.16 μmol DCPIP还原min−1 mg−1。分辨率为1.85 μ m的晶体结构揭示了活性位点中的La（III）离子，与MxaF中的钙离子相反。整体折叠类似于其他MDH结构，但5G-XoxF是溶液中的单体。通过生物层干涉法检测到5G-XoxF与其同源pMMO之间的相互作用，KD值为50 ± 17 μM。这些结果表明MDH-pMMO关联的替代模型，其中XoxF单体可以结合pMMO，并强调镧系元素依赖性MDH在生物甲烷氧化中的潜在重要性。
In methylotrophic bacteria, which use one-carbon (C1) compounds as a carbon source, methanol is oxidized by pyrroloquinoline quinone (PQQ)-dependent methanol dehydrogenase (MDH) enzymes. Methylotrophic genomes generally encode two distinct MDHs, MxaF and XoxF. MxaF is a well-studied, calcium-dependent heterotetrameric enzyme whereas XoxF is a lanthanide-dependent homodimer. Recent studies suggest that XoxFs are likely the functional MDHs in many environments. In methanotrophs, methylotrophs that utilize methane, interactions between particulate methane monooxygenase (pMMO) and MxaF have been detected. To investigate the possibility of interactions between pMMO and XoxF, XoxF was isolated from the methanotroph Methylomicrobium buryatense 5GB1C (5G-XoxF). Purified 5G-XoxF exhibits a specific activity of 0.16 μmol DCPIP reduced min−1 mg−1. The 1.85 Å resolution crystal structure reveals a La(III) ion in the active site, in contrast to the calcium ion in MxaF. The overall fold is similar to other MDH structures, but 5G-XoxF is a monomer in solution. An interaction between 5G-XoxF and its cognate pMMO was detected by biolayer interferometry, with a KD value of 50 ± 17 μM. These results suggest an alternative model of MDH-pMMO association, in which a XoxF monomer may bind to pMMO, and underscore the potential importance of lanthanide-dependent MDHs in biological methane oxidation.
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影响因子：
4.4
作者：
FASSEL, TA;BUCHHOLZ, LA;REMSEN, CC
通讯作者：
REMSEN, CC
影响因子：
14.9
作者：
Laskowski, RA
通讯作者：
Laskowski, RA
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
2.9
作者：
Culpepper, Megen A.;Rosenzweig, Amy C.
通讯作者：
Rosenzweig, Amy C.
影响因子：
2.8
作者：
Hibi, Yoshihisa;Asai, Kentaro;Kawai, Keiichi
通讯作者：
Kawai, Keiichi