Respiration and Heat Shock Protein After Short-Term Heating/Stretch-Fixing on Smooth Muscle Cells
Respiration and Heat Shock Protein After Short-Term Heating/Stretch-Fixing on Smooth Muscle Cells
复制标题
平滑肌细胞短期加热/拉伸固定后的呼吸和热休克蛋白
DOI：
10.1007/s13239-020-00458-y
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发表时间：
2020
影响因子：
1.8
通讯作者：
Nao Kaminota,Emiyu Ogawa,Hiroshi Kumagai,Kosuke Tsukada,Tsunenori Arai
中科院分区：
文献类型：
--
作者：
新井健太;櫻井遊;千田克幸;梅原健太;降幡知己;秋田英万;Nao Kaminota,Emiyu Ogawa,Hiroshi Kumagai,Kosuke Tsukada,Tsunenori Arai
目的外周动脉狭窄的治疗需要一种无支架的治疗装置。我们提出了短期加热球囊血管成形术，光热动力球囊血管成形术（PTDBA）。虽然在猪模型中PTDBA后的平滑肌细胞（SMC）被固定在拉伸的形态中，但尚未研究这种刺激对SMC的影响。血管扩张后平滑肌细胞迁移可能与慢性再狭窄有关。本研究的目的是检查呼吸活动和恢复能力的SMCs后，短期加热/stretch-fixingin vitro慢性期treatment effects discussion.MethodsSMCs在拉伸chamber加热15 s与拉伸和固定在拉伸形成。SMC迁移与细胞呼吸活动有关。使用用于呼吸活性评价的WST-8测定来测量ATP产生的量。细胞内的热休克蛋白70的表达测定通过ELISA的恢复能力evaluation.ResultsIn的情况下，60 °C加热，SMC呼吸活动后，短期加热/拉伸固定急剧下降，在所有的拉伸率。在50 °C加热的情况下，SMC呼吸活性先降低后升高。结论60 ° C拉伸条件下培养的SMC具有较高的恢复能力，但与SMC迁移相关的呼吸活性较低。这些结果可能是确定PTDBA治疗条件的重要依据。
PurposeA treatment device without a stent is needed for peripheral stenotic artery treatment. We have proposed short-term heating balloon angioplasty, photo-thermo dynamic balloon angioplasty (PTDBA). Though smooth muscle cells (SMCs) after PTDBA are fixed in a stretched formation in a porcine model, influences of this stimulus on SMCs have not been investigated. SMC migration after vascular dilatation would be related to chronic restenosis. The aim of this study was to examine respiratory activity and recovery ability of SMCs after short-term heating/stretch-fixingin vitrofor chronic phase treatment effect discussion.MethodsSMCs on a stretch chamber were heated for 15 s with stretching and fixed in a stretched formation. SMC migration is correlated with the cell respiratory activity. The amount of ATP production was measured using a WST-8 assay for respiratory activity evaluation. The intracellular expression of heat shock protein 70 was measured by an ELISA for recovery ability evaluation.ResultsIn the case of 60 °C heating, SMC respiratory activity after short-term heating/stretch-fixing decreased drastically in all stretching rates. In the case of 50 °C heating, SMC respiratory activity decreased and then increased. Alternatively, the recovery ability at 60 °C was greater than that at 50 °C.ConclusionsSMCs heated at 60 °C with stretching would have high recovery ability and low respiratory activity related to SMC migration. These results may be important evidence in determining the treatment condition in PTDBA.
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猪离体和体内模型短时加热扩张后血管壁平滑肌细胞的变形率
影响因子：
1.8
作者：
Mie Kunio;T. Arai
通讯作者：
T. Arai
[细胞增殖机制——细胞周期、癌基因和衰老]。
DOI：
10.1002/chin.200714268
发表时间：
2007
期刊：
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
影响因子：
--
作者：
T. Ide
通讯作者：
T. Ide
激光热球导管：具有短期加热时间的新型介入导管系统
DOI：
10.2530/jslsm1980.17.supplement_185
发表时间：
1996
期刊：
影响因子：
--
作者：
T. Arai;M. Yoshikawa;M. Kikuchi;Takayuki Miyake;M. Sakurada;K. Mizuno;A. Utsumi;K. Takeuchi
通讯作者：
K. Takeuchi
经皮冠状动脉腔内成形术再狭窄：激光球囊血管成形术的潜在预防。
DOI：
10.1016/0002-9149(87)90487-5
发表时间：
1987
期刊：
The American journal of cardiology
影响因子：
--
作者：
J. Spears
通讯作者：
J. Spears
激光球囊血管成形术：临床、血管造影和组织学结果。
影响因子：
24
作者：
Reis,GJ;Pomerantz,RM;Jenkins,RD;Kuntz,RE;Baim,DS;Diver,DJ;Schnitt,SJ;Safian,RD
通讯作者：
Safian,RD