Allele-specific expression at the androgen receptor alpha gene in a hybrid unisexual fish, the Amazon molly (Poecilia formosa).
Allele-specific expression at the androgen receptor alpha gene in a hybrid unisexual fish, the Amazon molly (Poecilia formosa).
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DOI：
10.1371/journal.pone.0186411
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Tiedemann R
中科院分区：
文献类型：
--
作者：
Zhu F;Schlupp I;Tiedemann R
全雌性亚马逊molly （Poecilia formosa）是大约12万年前大西洋molly （P. mexicana）和帆molly （P. latipinna）杂交的结果。作为雌雄同体的一种，福尔摩沙需要与异种雄虫交配，包括其雌雄同体祖先种的雄虫。然而，精子只能触发二倍体卵子的胚胎发生。精子捐献者的遗传信息通常不会对下一代的福尔摩沙做出贡献。因此，福尔摩沙在任何遗传位点一般都拥有一个来自其祖先物种的等位基因。这就提出了一个问题，即两个祖先等位基因是否在福尔摩沙中同样表达。等位基因特异性表达（ASE）已经在人类和鱼类等各种生物中进行了评估，ASE被发现在表型变异和疾病的背景下很重要。在这项研究中，我们利用Real-Time PCR技术估计了亚马逊河molis几种不同组织中雄激素受体α (arα)基因的ASE。我们在卵巢组织中发现了有利于母系祖先（墨西哥斑蝶）等位基因的等位基因。在鳃或脑组织中没有观察到这种等位基因偏向。两个等位基因的启动子区域测序揭示了已知CpG岛的Indel与差异表达之间的关联。未来的研究可能会揭示我们观察到的顺式调控差异是否是由卵巢特异性的反调控元件引起的，该元件优先激活母系祖先的等位基因。
The all-female Amazon molly (Poecilia formosa) is the result of a hybridization of the Atlantic molly (P. mexicana) and the sailfin molly (P. latipinna) approximately 120,000 years ago. As a gynogenetic species, P. formosa needs to copulate with heterospecific males including males from one of its bisexual ancestral species. However, the sperm only triggers embryogenesis of the diploid eggs. The genetic information of the sperm donor typically will not contribute to the next generation of P. formosa. Hence, P. formosa possesses generally one allele from each of its ancestral species at any genetic locus. This raises the question whether both ancestral alleles are equally expressed in P. formosa. Allele-specific expression (ASE) has been previously assessed in various organisms, e.g., human and fish, and ASE was found to be important in the context of phenotypic variability and disease. In this study, we utilized Real-Time PCR techniques to estimate ASE of the androgen receptor alpha (arα) gene in several distinct tissues of Amazon mollies. We found an allelic bias favoring the maternal ancestor (P. mexicana) allele in ovarian tissue. This allelic bias was not observed in the gill or the brain tissue. Sequencing of the promoter regions of both alleles revealed an association between an Indel in a known CpG island and differential expression. Future studies may reveal whether our observed cis-regulatory divergence is caused by an ovary-specific trans-regulatory element, preferentially activating the allele of the maternal ancestor.
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期刊：
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