Enhanced responsiveness to selective serotonin reuptake inhibitors during lactation.
Enhanced responsiveness to selective serotonin reuptake inhibitors during lactation.
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DOI：
10.1371/journal.pone.0117339
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Gregerson KA
中科院分区：
文献类型：
--
作者：
Jury NJ;McCormick BA;Horseman ND;Benoit SC;Gregerson KA
尽管产后抑郁症（PPD）是一种常见的病理学，但对产后情绪调节的生理学知之甚少。5-羟色胺能机制及其功能障碍被广泛认为是参与，这使得我们调查是否哺乳诱导中枢或外周5-羟色胺（5-HT）系统和相关的情感行为的变化。对哺乳期（产后第10天）和年龄匹配的未经产（非妊娠）C57 BL/6 J小鼠的脑切片进行5-HT免疫组织化学处理。测定5-HT免疫染色细胞总数和光密度。哺乳期小鼠表现出较低的免疫反应性5-HT和强度中缝背核相比，未经生产的控制。采用放射免疫分析法对哺乳期和未产小鼠血清5-HT进行定量。泌乳小鼠血清5-HT浓度高于未经产对照组。情感行为进行了评估，在哺乳期和非哺乳期女性产后10天，以及在未经生产的控制使用强迫游泳试验（FST）和大理石掩埋任务（MBT）。在前5天，动物接受选择性5-羟色胺再摄取抑制剂（SSRI，西酞普兰，5 mg/kg/天）或溶媒治疗。与未经产对照组相比，哺乳期小鼠在FST期间表现出较低的基线不动时间，并且在MBT期间埋藏较少的大理石。西酞普兰治疗改变了哺乳期小鼠的这些行为，进一步减少了FST期间的不动性和减少了大理石埋藏。相比之下，相同的西酞普兰治疗方案对产后非哺乳期或未经产女性的这些行为没有影响。我们的研究结果表明，在中枢和外周5-HT系统与哺乳期，独立于怀孕的变化。他们还证明了哺乳和SSRI治疗的反应性的显着相互作用，这对PPD的治疗具有重要意义。虽然最近的证据对SSRIs的有效性提出了质疑，但这些结果支持它们在治疗PPD中的治疗用途。
The physiology of mood regulation in the postpartum is poorly understood despite the fact that postpartum depression (PPD) is a common pathology. Serotonergic mechanisms and their dysfunction are widely presumed to be involved, which has led us to investigate whether lactation induces changes in central or peripheral serotonin (5-HT) systems and related affective behaviors. Brain sections from lactating (day 10 postpartum) and age-matched nulliparous (non-pregnant) C57BL/6J mice were processed for 5-HT immunohistochemistry. The total number of 5-HT immunostained cells and optical density were measured. Lactating mice exhibited lower immunoreactive 5-HT and intensity in the dorsal raphe nucleus when compared with nulliparous controls. Serum 5-HT was quantified from lactating and nulliparous mice using radioimmunoassay. Serum 5-HT concentrations were higher in lactating mice than in nulliparous controls. Affective behavior was assessed in lactating and non-lactating females ten days postpartum, as well as in nulliparous controls using the forced swim test (FST) and marble burying task (MBT). Animals were treated for the preceding five days with a selective serotonin reuptake inhibitor (SSRI, citalopram, 5mg/kg/day) or vehicle. Lactating mice exhibited a lower baseline immobility time during the FST and buried fewer marbles during the MBT as compared to nulliparous controls. Citalopram treatment changed these behaviors in lactating mice with further reductions in immobility during the FST and decreased marble burying. In contrast, the same regimen of citalopram treatment had no effect on these behaviors in either non-lactating postpartum or nulliparous females. Our findings demonstrate changes in both central and peripheral 5-HT systems associated with lactation, independent of pregnancy. They also demonstrate a significant interaction of lactation and responsiveness to SSRI treatment, which has important implications in the treatment of PPD. Although recent evidence has cast doubt on the effectiveness of SSRIs, these results support their therapeutic use in the treatment of PPD.
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DOI：
10.1016/j.ogc.2008.04.004
发表时间：
2008-09-01
影响因子：
3.2
作者：
Dossett, Emily C.
通讯作者：
Dossett, Emily C.
影响因子：
5.3
作者：
Bale, TL;Davis, AM;McCarthy, MM
通讯作者：
McCarthy, MM
影响因子：
17.7
作者：
Dietz, Patricia M.;Williams, Selvi B.;Hornbrook, Mark C.
通讯作者：
Hornbrook, Mark C.
影响因子：
6.6
作者：
COWEN, PJ;PARRYBILLINGS, M;NEWSHOLME, EA
通讯作者：
NEWSHOLME, EA
影响因子：
5.3
作者：
Cervo, L;Canetta, A;Invernizzi, RW
通讯作者：
Invernizzi, RW