Ester Linked Fatty Acid (ELFA) method should be used with caution for interpretating soil microbial communities and their relationships with environmental variables in forest soils.
Ester Linked Fatty Acid (ELFA) method should be used with caution for interpretating soil microbial communities and their relationships with environmental variables in forest soils.
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应谨慎使用酯连接脂肪酸 (ELFA) 方法来解释森林土壤中的土壤微生物群落及其与环境变量的关系
DOI：
10.1371/journal.pone.0251501
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Kang H
中科院分区：
文献类型：
--
作者：
Yu W;Gao H;Kang H
As an alternative for phospholipid fatty acid (PLFA) analysis, a simpler ester linked fatty acid (ELFA) analysis has been developed to characterize soil microbial communities. However, few studies have compared the two methods in forest soils where the contribution of nonmicrobial sources may be larger than that of microbial sources. Moreover, it remains unclear whether the two methods yield similar relationships of microbial biomass and composition with environmental variables. Here, we compared PLFA and ELFA methods with respect to microbial biomass and composition and their relationships with environmental variables in six oriental oak (Quercus variabilis) forest sites along a 1500-km latitudinal gradient in East China. We found that both methods had a low sample-to-sample variability and successfully separated overall community composition of sites. However, total, bacterial, and fungal biomass, the fungal-to-bacterial ratio, and the gram-positive to gram-negative bacteria ratio were not significantly or strongly correlated between the two methods. The relationships of these microbial properties with environmental variables (pH, precipitation, and clay) greatly differed between the two methods. Our study indicates that despite its simplicity, the ELFA method may not be as feasible as the PLFA method for investigating microbial biomass and composition and for identifying their dominant environmental drivers, at least in forest soils.
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DOI：
10.3791/54360
发表时间：
2016-08-26
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Quideau SA;McIntosh AC;Norris CE;Lloret E;Swallow MJ;Hannam K
通讯作者：
Hannam K
影响因子：
9.7
作者：
Drijber, RA;Doran, JW;Lyon, DJ
通讯作者：
Lyon, DJ
影响因子：
9.7
作者：
Drenovsky, RE;Elliott, GN;Scow, KM
通讯作者：
Scow, KM
影响因子：
9.7
作者：
ALMENDROS, G;SANZ, J
通讯作者：
SANZ, J
影响因子：
2.9
作者：
Schutter, ME;Dick, RP
通讯作者：
Dick, RP