Regulatory roles of ephrinA5 and its novel signaling pathway in mouse primary granulosa cell apoptosis and proliferation
Regulatory roles of ephrinA5 and its novel signaling pathway in mouse primary granulosa cell apoptosis and proliferation
复制标题
ephrinA5及其新信号通路对小鼠原代颗粒细胞凋亡和增殖的调节作用
DOI：
10.1080/15384101.2018.1456297
复制
发表时间：
2018-04
期刊：
影响因子：
4.3
通讯作者：
Yang Liguo
中科院分区：
文献类型：
--
作者：
Worku Tesfaye;Wang Kai;Ayers Duncan;Wu Di;Rehman Zia Ur;Zhou Hao;Yang Liguo
ABSTRACT Recent findings suggest that ephrinA5 (Efna5) has a novel role in female mouse fertility, in addition to its well-defined role as a neurogenesis factor. Nevertheless, its physiological roles in ovarian granulosa cells (GC) have not been determined. In this study, mouse GC were cultured and transfected with ephrin A5 siRNA and negative control to determine the effects of Efna5 on GC apoptosis, proliferation, cell cycle progression, and related signaling pathways. To understand the mode signaling, the mRNA expression levels of Efna5 receptors (Eph receptor A5, Eph receptor A3, Eph receptor A8, and Eph receptor B2) were examined. Both mRNA and protein expressions of apoptosis-related factors (Bax, Bcl-2, Caspase 8, Caspase 3, and Tnfα) and a proliferation marker, Pcna, were investigated. Additionally, the role of Efna5 on paracrine oocyte-secreted factors and steroidogenesis hormones were also explored. Efna5 silencing suppressed GC apoptosis by downregulating Bax and upregulating Bcl-2 in a Caspase 8-dependent manner. Efna5 knockdown promoted GC proliferation via p-Akt and p-ERK pathway activation. The inhibition of Efna5 enhanced BMH15 and estradiol expression, but suppressed GDF9, while progesterone level remained unaltered. These results demonstrated that Efna5 is a pro-apoptotic agent in GC and plays important role in folliculogenesis by mediating apoptosis, proliferation, and steroidogenesis in female mouse. Therefore Efna5 might be potential therapeutic target for female fertility disorders.
登录
查看更多内容
影响因子：
3.7
作者：
S. Westfall;I. Hendry;K. Obholz;B. Rueda;John S. Davis
通讯作者：
S. Westfall;I. Hendry;K. Obholz;B. Rueda;John S. Davis
Eph 受体和肝配蛋白作为癌症治疗的靶点
影响因子：
5.3
作者：
Xi HQ;Wu XS;Wei B;Chen L
通讯作者：
Chen L
影响因子：
78.5
作者：
Pasquale, Elena B.
通讯作者：
Pasquale, Elena B.
DOI：
10.1111/asj.12200
发表时间：
2014-06
期刊：
Animal science journal = Nihon chikusan Gakkaiho
影响因子：
--
作者：
Emori C;Sugiura K
通讯作者：
Sugiura K
影响因子：
1.7
作者：
A. Salustri;A. Camaioni;Cristina;D'Alessandris
通讯作者：
A. Salustri;A. Camaioni;Cristina;D'Alessandris