Adjuvanted pandemic influenza vaccine: variation of emulsion components affects stability, antigen structure, and vaccine efficacy.
Adjuvanted pandemic influenza vaccine: variation of emulsion components affects stability, antigen structure, and vaccine efficacy.
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辅助大流行性流感疫苗：乳液成分的变化会影响稳定性，抗原结构和疫苗功效。
DOI：
10.1111/irv.12031
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发表时间：
2013-09
影响因子：
4.4
通讯作者：
Baldwin SL
中科院分区：
文献类型：
--
作者：
Fox CB;Barnes V L;Evers T;Chesko JD;Vedvick TS;Coler RN;Reed SG;Baldwin SL
Fox等人（2012）：佐剂大流行性流感疫苗：乳剂成分的变化影响稳定性、抗原结构和疫苗效力。流感和其他呼吸道病毒DOI: 10.1111/irv.12031。背景佐剂配方是基于重组蛋白的现代疫苗的关键组成部分，如果没有额外的免疫刺激剂，这些疫苗的免疫原性通常很差。水包油乳剂包含一种先进的疫苗佐剂，是已批准的季节性和大流行性流感疫苗的成分。然而，系统评价不同乳剂组分体外稳定性和体内佐剂作用的报道很少。目的评价不同类型的表面活性剂、油和赋形剂在与重组H5N1抗原配制时对乳剂粒径稳定性、抗原结构相互作用和体内活性的影响。方法采用高压均质法制备乳剂，通过动态光散射、zeta电位、粘度、pH、溶血活性、电子显微镜、荧光光谱和SDS - PAGE等方法对乳剂单独或在疫苗抗原存在下进行表征。在小鼠模型中，通过测量抗体滴度、抗体分泌浆细胞、血凝抑制滴度和细胞因子的产生来表征疫苗的体内活性。结果表明，表面活性剂的种类和附加赋形剂的存在对生物活性的影响并不重要，而油的结构对生物活性的影响至关重要。此外，我们报告说，简化的双组分乳剂在粒度上比更复杂的配方更稳定。最后，抗原结构与各种乳剂相互作用的差异似乎与体内活性无关。结论水包油乳剂可显著增强对大流行性流感抗原的抗体和细胞免疫反应。基于角鲨烯的乳剂和基于中链甘油三酯的乳剂之间佐剂活性的巨大差异主要是由于油成分的生物活性，而不是抗原与乳剂的物理相互作用。
Please cite this paper as: Fox et al. (2012) Adjuvanted pandemic influenza vaccine: variation of emulsion components affects stability, antigen structure, and vaccine efficacy. Influenza and Other Respiratory Viruses DOI: 10.1111/irv.12031. Abstract Background Adjuvant formulations are critical components of modern vaccines based on recombinant proteins, which are often poorly immunogenic without additional immune stimulants. Oil‐in‐water emulsions comprise an advanced class of vaccine adjuvants that are components of approved seasonal and pandemic influenza vaccines. However, few reports have been published that systematically evaluate the in vitro stability and in vivo adjuvant effects of different emulsion components. Objectives To evaluate distinct classes of surfactants, oils, and excipients, for their effects on emulsion particle size stability, antigen structural interactions, and in vivo activity when formulated with a recombinant H5N1 antigen. Methods Emulsions were manufactured by high pressure homogenization and characterized alone or in the presence of vaccine antigen by dynamic light scattering, zeta potential, viscosity, pH, hemolytic activity, electron microscopy, fluorescence spectroscopy, and SDS‐PAGE. In vivo vaccine activity in the murine model was characterized by measuring antibody titers, antibody‐secreting plasma cells, hemagglutination inhibition titers, and cytokine production. Results We demonstrate that surfactant class and presence of additional excipients are not critical for biological activity, whereas oil structure is crucial. Moreover, we report that simplified two‐component emulsions appear more stable by particle size than more complex formulations.Finally, differences in antigen structural interactions with the various emulsions do not appear to correlate with in vivo activity. Conclusions Oil‐in‐water emulsions can significantly enhance antibody and cellular immune responses to a pandemic influenza antigen. The dramatic differences in adjuvant activity between squalene‐based emulsion and medium chain triglyceride‐based emulsion are due principally to the biological activity of the oil composition rather than physical interactions of the antigen with the emulsion.
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影响因子：
5.5
作者：
Morel, Sandra;Didierlaurent, Arnaud;Van Mechelen, Marcelle
通讯作者：
Van Mechelen, Marcelle
DOI：
10.3390/molecules14093286
发表时间：
2009-09-01
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
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