Polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells.
Polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells.
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DOI：
10.1007/s00011-010-0242-0
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发表时间：
2011-02
影响因子：
6.7
通讯作者：
Ebersole, J. L.
中科院分区：
文献类型：
--
作者：
Huang, C. B.;Altimova, Y.;Strange, S.;Ebersole, J. L.
研究牙周病的多微生物感染，引起局部牙龈组织的炎症介质/细胞因子/趋化因子，以及粘膜表面抗原呈递细胞（如巨噬细胞和树突状细胞（dc））的多细菌攻击。研究了人巨噬细胞和dc在多细菌攻击下的细胞因子/趋化因子谱。与革兰氏阳性菌相比，口腔革兰氏阴性菌从巨噬细胞中诱导的IL-8水平明显更高。革兰氏阳性菌对巨噬细胞诱导趋化因子无协同作用。相反，对口腔革兰氏阴性菌诱导巨噬细胞协同产生IL-8。tnf - α则没有类似的结果，它只在多菌攻毒中出现。选择的革兰氏阴性细菌对在未成熟树突状细胞中协同产生IL-6。在成熟的dc中，牙龈卟啉单胞菌/核梭杆菌和中间卟啉单胞菌/F。核仁多菌侵染对IL-6和tnf - α水平有显著的增效作用。然而，只有Pi/Fn组合协同产生IL-12，并且mDCs对IL-10的产生似乎没有多菌效应。这些结果表明，连接先天免疫反应和适应性免疫反应的细胞的多细菌挑战导致不同的反应概况，这取决于作为多细菌复合体组成部分的微生物的特征。
To investigate the polymicrobial infection of periodontal disease, which elicits inflammatory mediators/cytokines/chemokines in the local gingival tissues, and a polybacterial challenge of antigen-presenting cells, e.g. macrophages and dendritic cells (DCs), at the mucosal surface. The cytokine/chemokine profiles of human macrophages and DCs in response to polybacterial challenges were investigated. Oral Gram-negative bacteria elicited significantly greater IL-8 levels from macrophages, compared to Gram-positive bacteria. Gram-positive bacteria did not show synergism in inducing this chemokine from macrophages. In contrast, pairs of oral Gram-negative bacteria elicited synergistic production of IL-8 by macrophages. Similar results were not observed with TNFα, which only appeared additive with the polybacterial challenge. Selected Gram-negative bacterial pairs synergized in IL-6 production by immature DCs. In mature DCs (mDCs), a Porphyromonas gingivalis/Fusobacterium nucleatum and Porphyromonas intermedia/F. nucleatum polybacterial challenge resulted in significant synergism for IL-6 and TNFα levels. However, only the Pi/Fn combination synergized for IL-12 production and there appeared to be no polybacterial effect on IL-10 production by the mDCs. These results indicate that a polybacterial challenge of cells linking innate and adaptive immune responses results in varied response profiles that are dependent upon the characteristics of the microorganisms that are components of the polybacterial complex.
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影响因子：
6.7
作者：
Dye, BA;Choudhary, K;Papapanou, PN
通讯作者：
Papapanou, PN
影响因子：
2
作者：
Ebersole, JL;Cappelli, D;Holt, SC
通讯作者：
Holt, SC
影响因子：
4.3
作者：
HAFSTROM, CA;WIKSTROM, MB;DAHLEN, GG
通讯作者：
DAHLEN, GG
影响因子：
2.5
作者：
Cario, Elke
通讯作者：
Cario, Elke
影响因子：
4.4
作者：
Harokopakis, Evlambia;Albzreh, Mohamad H.;Hajishengallis, George
通讯作者：
Hajishengallis, George