Differential Post-Translational Modifications of Proteins in Bladder Ischemia.
Differential Post-Translational Modifications of Proteins in Bladder Ischemia.
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DOI：
10.3390/biomedicines12010081
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发表时间：
2023-12-28
期刊：
影响因子：
4.7
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中科院分区：
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临床和基础研究表明，膀胱缺血可能是下尿路症状（LUTS）发展的一个独立变量。我们已经报道了膀胱的缺血性变化涉及蛋白质功能域的差异表达和翻译后修饰（PTMs）。在本研究中，我们对先前报道的蛋白质组学数据集进行了深入分析，以进一步表征膀胱缺血中的PTMs蛋白。我们对膀胱缺血中蛋白质的蛋白质组学分析检测到可能由PTMs导致的非编码氨基酸（ncAAs）的差异形成。深入分析发现，膀胱蛋白质组中的三组蛋白，包括收缩蛋白及其相关蛋白、应激反应蛋白和细胞信号相关蛋白，明显受到缺血的影响。缺血对蛋白质的不同PTMs似乎影响膀胱中重要的信号通路，并引起应激反应、收缩和细胞信号相关蛋白翻译后结构完整性的关键变化。我们的数据表明，蛋白质的不同PTMs可能在细胞应激的发展、平滑肌细胞对收缩刺激的敏感化以及膀胱缺血中的顺从细胞信号传导中发挥作用。这些观察结果可能为未来的研究提供基础，以验证和定义修饰的生物标志物的临床翻译，以精确诊断膀胱功能障碍和开发新的治疗靶点。
Clinical and basic research suggests that bladder ischemia may be an independent variable in the development of lower urinary tract symptoms (LUTS). We have reported that ischemic changes in the bladder involve differential expression and post-translational modifications (PTMs) of the protein’s functional domains. In the present study, we performed in-depth analysis of a previously reported proteomic dataset to further characterize proteins PTMs in bladder ischemia. Our proteomic analysis of proteins in bladder ischemia detected differential formation of non-coded amino acids (ncAAs) that might have resulted from PTMs. In-depth analysis revealed that three groups of proteins in the bladder proteome, including contractile proteins and their associated proteins, stress response proteins, and cell signaling-related proteins, are conspicuously impacted by ischemia. Differential PTMs of proteins by ischemia seemed to affect important signaling pathways in the bladder and provoke critical changes in the post-translational structural integrity of the stress response, contractile, and cell signaling-related proteins. Our data suggest that differential PTMs of proteins may play a role in the development of cellular stress, sensitization of smooth muscle cells to contractile stimuli, and deferential cell signaling in bladder ischemia. These observations may provide the foundation for future research to validate and define clinical translation of the modified biomarkers for precise diagnosis of bladder dysfunction and the development of new therapeutic targets against LUTS.
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DOI：
10.1126/science.1260793
发表时间：
2015-02-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Battle A;Khan Z;Wang SH;Mitrano A;Ford MJ;Pritchard JK;Gilad Y
通讯作者：
Gilad Y
前列腺钬激光去核术后膀胱血流增加对储尿症状的影响
影响因子：
3.7
作者：
Saito K;Hisasue S;Ide H;Aoki H;Muto S;Yamaguchi R;Tsujimura A;Horie S
通讯作者：
Horie S
影响因子：
6.6
作者：
IRWIN, P;GALLOWAY, NTM
通讯作者：
GALLOWAY, NTM
影响因子：
3.5
作者：
De, Elise J. B.;Hou, Ping;Westney, O. Lenaine
通讯作者：
Westney, O. Lenaine
DOI：
10.1016/j.bbapap.2021.140683
发表时间：
2021-09
期刊：
Biochimica et biophysica acta. Proteins and proteomics
影响因子：
--
作者：
通讯作者：
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