Sensitive immunosensor for cancer biomarker based on dual signal amplification strategy of graphene sheets and multienzyme functionalized carbon nanospheres.
Sensitive immunosensor for cancer biomarker based on dual signal amplification strategy of graphene sheets and multienzyme functionalized carbon nanospheres.
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DOI：
10.1021/ac100036p
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发表时间：
2010-04-01
影响因子：
7.4
通讯作者：
Lin, Yuehe
中科院分区：
文献类型：
--
作者：
Du, Dan;Zou, Zhexiang;Shin, Yongsoon;Wang, Jun;Wu, Hong;Engelhard, Mark H.;Liu, Jun;Aksay, Ilhan A.;Lin, Yuehe
描述了一种新型的用于癌症生物标志物α-胎蛋白（AFP）敏感检测的电化学免疫传感器，该传感器使用石墨烯片传感器平台和辣根过氧化物酶二抗（HRP-Ab2）标记的功能化碳纳米球（CNSs）。基于双重信号放大策略，大大提高了对癌症生物标志物的敏感性：首先，合成的CNSs产生均匀而狭窄的尺寸分布，这使得每个纳米球上都可以发生HRP-Ab2的几个结合事件。通过“三明治”免疫反应将HRP-Ab2-CNSs的多生物偶联物引入电极表面，提高了灵敏度。其次，用于生物传感器平台的功能化石墨烯片增加了捕获大量一抗（Ab1）的表面积，从而放大了检测响应。基于石墨烯片的双信号放大策略和多酶标记，所开发的免疫传感器的检测信号比未修饰石墨烯和CNSs标记的免疫传感器提高了7倍。该方法可在0.05 ~ 6 ng mL-1的线性校准范围内响应0.02 ng mL-1的AFP。这种扩增策略是临床筛查癌症生物标志物和即时诊断的一个很有前途的平台。
A novel electrochemical immunosensor for sensitive detection of cancer biomarker α-fetoprotein (AFP) is described that uses a graphene sheet sensor platform and functionalized carbon nanospheres (CNSs) labeling with horseradish peroxidase-secondary antibodies (HRP-Ab2). Greatly enhanced sensitivity for the cancer biomarker is based on a dual signal amplification strategy: first, the synthesized CNSs yielded a homogeneous and narrow size distribution, which allowed several binding events of HRP-Ab2 on each nanosphere. Enhanced sensitivity was achieved by introducing the multi-bioconjugates of HRP-Ab2-CNSs onto the electrode surface through “sandwich” immunoreactions. Secondly, functionalized graphene sheets used for the biosensor platform increased the surface area to capture a large amount of primary antibodies (Ab1), thus amplifying the detection response. On the basis of the dual signal amplification strategy of graphene sheets and the multi-enzyme labeling, the developed immunosensor showed a 7-fold increase in detection signal compared to the immunosensor without graphene modification and CNSs labeling. The proposed method could respond to 0.02 ng mL-1 AFP with a linear calibration range from 0.05 to 6 ng mL-1. This amplification strategy is a promising platform for clinical screening of cancer biomarkers and point-of-care diagnostics.
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影响因子：
17.1
作者：
Mani, Vigneshwaran;Chikkaveeraiah, Bhaskara V.;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
5.4
作者：
Li, Yueming;Tang, Longhua;Li, Jinghong
通讯作者：
Li, Jinghong
影响因子：
6.1
作者：
Cesaro-Tadic, S;Dernick, G;Delamarche, E
通讯作者：
Delamarche, E
影响因子：
7.4
作者：
Authier, L;Grossiord, C;Limoges, B
通讯作者：
Limoges, B
影响因子：
2.2
作者：
Fu, Zhifeng;Hao, Chen;Ju, Huangxian
通讯作者：
Ju, Huangxian