The S2 glycoprotein subunit of porcine epidemic diarrhea virus contains immunodominant neutralizing epitopes.
The S2 glycoprotein subunit of porcine epidemic diarrhea virus contains immunodominant neutralizing epitopes.
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DOI：
10.1016/j.virol.2017.06.013
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发表时间：
2017-09
期刊：
影响因子：
3.7
通讯作者：
Diel DG
中科院分区：
文献类型：
--
作者：
Okda FA;Lawson S;Singrey A;Nelson J;Hain KS;Joshi LR;Christopher-Hennings J;Nelson EA;Diel DG
猪流行性腹泻病毒（PEDV）刺突蛋白（S）是抗PEDV中和抗体的主要靶点。在此，使用一组S特异性单克隆抗体（mAb）鉴定PEDV的免疫显性中和表位。11个S特异性mAb中的10个成功地在体外中和PEDV感染性。值得注意的是，通过肽ELISA进行的表位作图显示，这些mAb中的9种识别位于S2糖蛋白亚基的N-末端的线性中和表位（氨基酸[aa] 744-759、747-774和/或756-771）。此外，一种mAb识别位于S2 C-末端的中和表位（aa 1371-1377），而仅一种中和mAb与S1糖蛋白亚基区域（aa 499-600）反应。值得注意的是，识别S2亚基内的表位的mAb呈现针对PEDV的最高中和活性。总之，这些结果表明，S2糖蛋白亚基含有主要的抗原决定簇，也许，免疫显性中和表位的PEDV。开发并表征了一组PEDV S特异性中和抗体。S-特异性中和mAb在体外呈现针对PEDV的有效中和活性。表位作图显示，大多数中和mAb靶向S2蛋白亚基的N-末端。S2亚基似乎含有PEDV的免疫显性中和表位。
The porcine epidemic diarrhea virus (PEDV) spike (S) protein is the major target of neutralizing antibodies against PEDV. Here immunodominant neutralizing epitopes of PEDV were identified using a panel of S-specific monoclonal antibodies (mAbs). Ten of eleven S-specific mAbs successfully neutralized PEDV infectivity in vitro. Notably, epitope mapping by peptide ELISAs revealed that nine of these mAbs recognized linear neutralizing epitopes located in the N-terminus of the S2 glycoprotein subunit (amino acids [aa] 744–759, 747–774 and/or 756–771). Additionally, one mAb recognized a neutralizing epitope located in the C-terminus of S2 (aa 1371–1377), while only one neutralizing mAb reacted against a region of the S1 glycoprotein subunit (aa 499–600). Notably, mAbs that recognized epitopes within the S2 subunit presented the highest neutralizing activity against PEDV. Together these results indicate that the S2 glycoprotein subunit contains major antigenic determinants and, perhaps, the immunodominant neutralizing epitopes of PEDV. A panel of PEDV S-specific neutralizing antibodies were developed and characterized. S-specific neutralizing mAbs presented potent neutralizing activity against PEDV in vitro. Epitope mapping revealed that most neutralizing mAbs target the N-terminus of the S2 protein subunit. S2 subunit seems to contain immunodominant neutralizing epitopes of PEDV.
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