Impact of taurine on red blood cell metabolism and implications for blood storage.
Impact of taurine on red blood cell metabolism and implications for blood storage.
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DOI：
10.1111/trf.15810
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发表时间：
2020-06
期刊：
影响因子：
2.9
通讯作者：
D'Alessandro A
中科院分区：
文献类型：
--
作者：
Bertolone L;Roy MK;Hay AM;Morrison EJ;Stefanoni D;Fu X;Kanias T;Kleinman S;Dumont LJ;Stone M;Nemkov T;Busch MP;Zimring JC;D'Alessandro A
牛磺酸是一种抗氧化剂，在一些常见的能量饮料中含量丰富。在这里，我们假设红细胞（RBC）中牛磺酸的抗氧化活性可以用来抵消储存引起的氧化应激。对健康志愿者 (n = 4) 的基线血浆和红细胞进行代谢组学分析，并在饮用一整罐富含牛磺酸的常见能量饮料（1000 毫克/份）后进行代谢组学分析。还原性研究还通过将人红细胞与牛磺酸（未标记或 13C15N 标记）以递增剂量（0、100、500 和 1000 μmol/L）在 37°C 下孵育长达 16 小时，并进行或不进行过氧化氢（0.1% 或 0.5%）氧化应激挑战。最后，在代谢组学和输血后恢复研究之前，我们在血库条件下将人类和小鼠红细胞储存在添加有 500 μmol/L 牛磺酸的添加剂中。饮用能量饮料会增加血浆和红细胞中牛磺酸的水平，同时红细胞中糖酵解和谷胱甘肽 (GSH) 代谢也会增加。在与牛磺酸和过氧化氢一起孵育后，在体外重现了这些观察结果。来自 REDS-III RBC-Omics 供体生物库的红细胞中的牛磺酸水平与 GSH 和谷胱甘肽代谢物的总水平成正比，与氧化溶血测量值成反比。在存在牛磺酸的情况下储存人红细胞可改善储存质量的能量和氧化还原标记，并提高 FVB 小鼠的输血后恢复率。牛磺酸在体内和体外调节红细胞抗氧化代谢，观察到与输血医学的潜在相关性。
Taurine is an antioxidant that is abundant in some common energy drinks. Here we hypothesized that the antioxidant activity of taurine in red blood cells (RBCs) could be leveraged to counteract storage-induced oxidant stress. Metabolomics analyses were performed on plasma and RBCs from healthy volunteers (n = 4) at baseline and after consumption of a whole can of a common, taurine-rich (1000 mg/serving) energy drink. Reductionistic studies were also performed by incubating human RBCs with taurine ex vivo (unlabeled or 13C15N-labeled) at increasing doses (0, 100, 500, and 1000 μmol/L) at 37°C for up to 16 hours, with and without oxidant stress challenge with hydrogen peroxide (0.1% or 0.5%). Finally, we stored human and murine RBCs under blood bank conditions in additives supplemented with 500 μmol/L taurine, before metabolomics and posttransfusion recovery studies. Consumption of energy drinks increased plasma and RBC levels of taurine, which was paralleled by increases in glycolysis and glutathione (GSH) metabolism in the RBC. These observations were recapitulated ex vivo after incubation with taurine and hydrogen peroxide. Taurine levels in the RBCs from the REDS-III RBC-Omics donor biobank were directly proportional to the total levels of GSH and glutathionylated metabolites and inversely correlated to oxidative hemolysis measurements. Storage of human RBCs in the presence of taurine improved energy and redox markers of storage quality and increased posttransfusion recoveries in FVB mice. Taurine modulates RBC antioxidant metabolism in vivo and ex vivo, an observation of potential relevance to transfusion medicine.
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影响因子：
2.9
作者：
D'Alessandro A;Nemkov T;Kelher M;West FB;Schwindt RK;Banerjee A;Moore EE;Silliman CC;Hansen KC
通讯作者：
Hansen KC
影响因子：
7.1
作者：
LAIDLAW, SA;SHULTZ, TD;KOPPLE, JD
通讯作者：
KOPPLE, JD
影响因子：
2.9
作者：
D'Alessandro, Angelo;Culp-Hill, Rachel;Cristman, A. M.
通讯作者：
Cristman, A. M.
影响因子：
10.1
作者：
de Wolski, Karen;Fu, Xiaoyoun;Zimring, James C.
通讯作者：
Zimring, James C.
影响因子：
2.9
作者：
Endres-Dighe, Stacy M.;Guo, Yuelong;Cristman, A. M.
通讯作者：
Cristman, A. M.