Routine storage of red blood cell (RBC) units in additive solution-3: a comprehensive investigation of the RBC metabolome.
Routine storage of red blood cell (RBC) units in additive solution-3: a comprehensive investigation of the RBC metabolome.
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DOI：
10.1111/trf.12975
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发表时间：
2015-06
期刊：
影响因子：
2.9
通讯作者：
Hansen KC
中科院分区：
文献类型：
--
作者：
D'Alessandro A;Nemkov T;Kelher M;West FB;Schwindt RK;Banerjee A;Moore EE;Silliman CC;Hansen KC
在大多数国家，浓缩红细胞 (RBC) 可以在输血前保存长达 42 天。然而，观察性研究表明，储存时间可能与发病率和死亡率增加有关。虽然临床试验正在进行中，但储存在多种添加剂溶液中的红细胞的代谢受损已被记录。在这里，我们假设，尽管有报道的有益效果，但在添加剂溶液 3 (AS-3) 中储存会导致在单位保质期结束前几周出现代谢障碍。在第 0 天的白细胞去除之前、期间和之后对 5 个白细胞过滤的 AS-3 RBC 单位进行取样，然后从储存第 1 天到第 42 天每周进行一次测定。使用 UHPLC-MS 代谢组学工作流程对红细胞提取物和上清液进行分析。血库储存显着影响第 14 天红细胞提取物和上清液的代谢特征。除了能量和氧化还原代谢受损之外，还观察到细胞内和细胞外的氨基酸积累与储存时间成正比，这表明谷氨酰胺和丝氨酸代谢在老化红细胞中发挥着作用。储存红细胞的代谢组学可以推动替代添加剂解决方案的引入，以解决本文报道的一些储存依赖性代谢病变，从而提高输注红细胞的质量并最大限度地减少与患者发病率的潜在联系。
In most countries, packed red blood cells (RBCs) can be stored up to 42 days before transfusion. However, observational studies have suggested that storage duration might be associated with increased morbidity and mortality. While clinical trials are underway, impaired metabolism has been documented in RBCs stored in several additive solutions. Here we hypothesize that, despite reported beneficial effects, storage in additive solution-3 (AS-3) results in metabolic impairment weeks before the end of the unit shelf-life. Five leukocyte-filtered AS-3 RBC units were sampled before, during and after leukoreduction at day0, and then assayed on a weekly basis from storage day1 through day42. RBC extracts and supernatants were assayed using a UHPLC-MS metabolomics workflow. Blood bank storage significantly affects metabolic profiles of RBC extracts and supernatants by day14. In addition to energy and redox metabolism impairment, intra- and extracellular accumulation of amino acids was observed proportionally to storage duration, suggesting a role for glutamine and serine metabolism in aging RBCs. Metabolomics of stored RBCs could drive the introduction of alternative additive solutions to address some of the storage-dependent metabolic lesions herein reported, thereby increasing the quality of transfused RBCs and minimizing potential links to patient morbidity.
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