Synthesis and characterization of thermoresponsive polyamidoamine-polyethylene glycol-poly(D,L-lactide) core-shell nanoparticles.
Synthesis and characterization of thermoresponsive polyamidoamine-polyethylene glycol-poly(D,L-lactide) core-shell nanoparticles.
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DOI：
10.1016/j.actbio.2009.08.036
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发表时间：
2010-03
影响因子：
9.7
通讯作者：
Yang, Hu
中科院分区：
文献类型：
--
作者：
Kailasan, Arunvel;Yuan, Quan;Yang, Hu
本工作描述了新型温敏性高度支化聚酰胺胺-聚乙二醇-聚（D，L-丙交酯）（PAMAM-PEG-PDLLA）核壳纳米粒子的合成和表征。通过将不同链长（1500、6000和12000 g/mol）的PEG与聚酰胺-胺（PAMAM）树枝状大分子G3.0偶联，然后开环聚合DLLA，合成了一系列树枝状PEG-PDLLA纳米粒。采用茚三酮法、核磁共振氢谱、红外光谱、动态光散射、原子力显微镜对纳米粒子的结构和组成进行了表征。用紫外-可见分光光度法研究了PEG-PDLLA水溶液的溶胶-凝胶相变。根据我们的研究结果，树枝状PEG-PDLLA纳米粒子在水溶液中可以自组装成亚微米/微米聚集体，聚集体的大小取决于温度和PEG-PDLLA链长。此外，树枝状PEG-PDLLA溶液随着温度的升高表现出溶胶-凝胶相变。所构建的树枝状PEG-PDLLA纳米粒具有高的细胞相容性，这是显着改善相比，PAMAM树枝状聚合物。证明了树枝状PEG-PDLLA纳米粒用于包封水不溶性药物如喜树碱的潜力。我们开发的树枝状PEG-PDLLA纳米颗粒提供了更大的结构灵活性，并为药物递送提供了一种新型的纳米结构温敏载体。
This work describes the synthesis and characterization of novel thermoresponsive highly-branched polyamidoamine-polyethylene glycol-poly (D, L-lactide) (PAMAM-PEG-PDLLA) core-shell nanoparticles. A series of dendritic PEG-PDLLA nanoparticles were synthesized through conjugation of PEG of various chain lengths (1500, 6000, and 12000 g/mol) to polyamidoamine (PAMAM) dendrimer G3.0 and subsequent ring-opening polymerization of DLLA. Ninhydrin assay, 1H-NMR, FT-IR, dynamic light scattering, AFM were used to characterize the structure and compositions of dendritic PEG-PDLLA nanoparticles. Sol-gel phase transition of aqueous dendritic PEG-PDLLA solutions was measured using UV-Vis spectroscocopy. According to our results, dendritic PEG-PDLLA nanoparticles in aqueous solutions can self-assemble into sub-micron/micron aggregates and the size of aggregates is dependent on temperature and PEG-PDLLA chain length. Further, dendritic PEG-PDLLA solutions exhibit sol-gel phase transition with increasing temperature. The constructed dendritic PEG-PDLLA nanoparticles possess high cytocompatibility, which is significantly improved as compared to PAMAM dendrimers. The potential of dendritic PEG-PDLLA nanoparticles for encapsulation of water insoluble drugs such as camptothecin was demonstrated. Dendritic PEG-PDLLA nanoparticles we developed offer greater structural flexibility and provide a novel nanostructured thermoresponsive carrier for drug delivery.
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