pol-miR-7133 and pol-miR-3p-9227 of Japanese flounder Paralichthys olivaceus modulate Streptococcus iniae infection through regulation of the common target gene LAMP2
pol-miR-7133 and pol-miR-3p-9227 of Japanese flounder Paralichthys olivaceus modulate Streptococcus iniae infection through regulation of the common target gene LAMP2
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日本牙鲆的 pol-miR-7133 和 pol-miR-3p-9227 通过调节共同靶基因 LAMP2 调节海豚链球菌感染
DOI：
10.1016/j.aquaculture.2020.734980
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发表时间：
2020-04
期刊：
影响因子：
4.5
通讯作者：
Sun Li
中科院分区：
文献类型：
--
作者：
Sun Yanling;Liu Shuang;Sun Li
微小RNA（microRNAs，miRNAs）是一类非编码的内源性小分子RNA，通过调控靶基因的表达在多种细胞过程中发挥重要作用。在本研究中，我们鉴定了两种日本比目鱼（Paralichthys olivaceus）miRNAs，即，pol-miR-7133和pol-miR-3 p-9227，并检测了与海豚链球菌感染相关的miRNAs及其靶基因的功能。pol-miR-7133和pol-miR-3 p-9227被鉴定为在海豚链球菌感染过程中下调的miRNA。这两种miRNAs均通过与比目鱼溶酶体相关膜蛋白2（lysosome-associated membrane protein 2，LAMP 2）亚型3（PoLAMP 23）的3′-非翻译区（3 '-UTR）相互作用靶向该基因。体外研究表明PoLAMP 2 -3表达与pol-miR-7133和pol-miR-3 p-9227表达呈负相关。由于迄今为止还没有关于鱼类LAMP 2的研究文献，我们研究了PoLAMP 2 -3的生物学特性。我们发现PoLAMP 2 -3表达发生在多种组织中，并且在肝脏中最丰富。PoLAMP 2 -3是一种强酸性跨膜蛋白，定位于溶酶体和细胞膜，体外研究表明，过表达pol-miR-7133和pol-miR-3 p-9227或干扰PoLAMP 2 -3的表达可显著增强海豚链球菌对比目鱼细胞的感染。同时，体内研究表明，敲低pol-miR-7133和pol-miR-3 p-9227或过表达PoLAMP 2 -3可抑制S.在比目鱼中，PoLAMP 2 -3的敲低促进了S.感染。总之，这些结果首次揭示了鱼类LAMP 2的重要免疫功能，并表明pol-miR-7133和pol-miR-3 p-9227通过LAMP 2在鱼类的抗菌免疫中发挥重要作用。
MicroRNAs (miRNAs) are small, non-coding endogenous RNAs that play critical roles in diverse cellular processes through regulating target gene expression. In the present study, we identified two Japanese flounder (Paralichthys olivaceus) miRNAs,i.e., pol-miR-7133 and pol-miR-3p-9227, and examined the functions of the miRNAs and their target gene in association with the infection of Streptococcus iniae. pol-miR-7133 and pol-miR-3p-9227 were identified as miRNAs downregulated duringS.iniae infection. Both miRNAs targeted the gene of lysosome-associated membrane protein 2 (LAMP2) isoform 3 of flounder (named PoLAMP23) by interaction with the 3′-untranslated region (3’-UTR) of PoLAMP2–3. Bothin vivoandin vitrostudies showed that PoLAMP2–3 expression negatively correlated with pol-miR-7133 and pol-miR-3p-9227 expression. Since to date no study on fish LAMP2 had been documented, we investigated the biological property of PoLAMP2–3. We found that PoLAMP2–3 expression occurred in multiple tissues and was most abundant in liver. PoLAMP2–3 is a highly acidic transmembrane protein and was observed to localize in both lysosome and cellular membrane.In vitrostudies showed that overexpression of pol-miR-7133 and pol-miR-3p-9227 or interference with PoLAMP2-3 expression significantly enhancedS.iniaeinfection in flounder cells. Consistently,in vivostudies showed that knockdown of pol-miR-7133 and pol-miR-3p-9227, or overexpression of PoLAMP2-3, inhibitedS. iniaeinfection in flounder, whereas knockdown of PoLAMP2-3 in flounder facilitatedS. iniaeinfection. Together these results revealed for the first time an important immune function of fish LAMP2 and indicated that pol-miR-7133 and pol-miR-3p-9227,viaLAMP2, play a vital role in anti-bacterial immunity in fish.
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影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
日本牙鲆 (Paralichthys olivaceus) 的 pol-miR-194a 抑制 I 型干扰素反应并促进迟缓爱德华氏菌感染
影响因子：
4.7
作者：
Guan, Xiao-lu;Zhang, Bao-cun;Sun, Li
通讯作者：
Sun, Li
影响因子：
3.7
作者：
Fu Y;Shi Z;Wu M;Zhang J;Jia L;Chen X
通讯作者：
Chen X
DOI：
--
发表时间：
2015
期刊：
Journal of Northeast Agricultural University
影响因子：
--
作者：
Xue Xiaol
通讯作者：
Xue Xiaol
影响因子：
13.3
作者：
Fujiwara Y;Kikuchi H;Aizawa S;Furuta A;Hatanaka Y;Konya C;Uchida K;Wada K;Kabuta T
通讯作者：
Kabuta T