Cytokine profiles among patients co-infected with Plasmodium falciparum malaria and soil borne helminths attending Kampala International University Teaching Hospital, in Uganda.
Cytokine profiles among patients co-infected with Plasmodium falciparum malaria and soil borne helminths attending Kampala International University Teaching Hospital, in Uganda.
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DOI：
10.1186/s13223-018-0235-z
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Mwandah DC
中科院分区：
文献类型：
--
作者：
Bwanika R;Kato CD;Welishe J;Mwandah DC
疟疾和蠕虫在热带非洲有着相同的地理分布。关于蠕虫和疟疾在人类中共同感染的相互作用的研究很少，主要是流行病学研究，关于细胞免疫反应的信息很少。本研究旨在确定在坎帕拉国际大学教学医院（KIU）就诊的合并感染恶性疟原虫疟疾和土源性蠕虫的患者的细胞因子谱。在KIU教学医院招募了240名患者进行病例对照研究。恶性疟55例（22.9%），土源性蠕虫63例（26.3%）。对照组89例（37.1%），恶性疟原虫疟疾和土源性蠕虫共感染组33例（13.8%）。病例被定义为恶性疟原虫疟疾血涂片阳性、有蠕虫或两者合并感染的病例。阴性对照是恶性疟原虫疟疾血涂片阴性的那些和没有粪便寄生虫感染的那些。本研究招募了表现出疟疾体征和症状的患者或怀疑有蠕虫的患者。从患有和不患有恶性疟原虫疟疾的患者、患有和不患有蠕虫的患者的血浆样品中检测一组五种细胞因子（IFN-γ、TNF-α、IL-6、TGF-β和IL-10），然后使用10% Giemsa染色的厚血涂片对合并感染两种疾病的患者进行诊断，并按照标准程序按照Kato Katz技术进行粪便检查。男女性恶性疟患病率分别为28例（11.7%）和27例（11.3%）。土源性蠕虫的总患病率为26.3%，其中窝藏蠕虫，13.8%的恶性疟原虫合并感染。恶性疟原虫疟疾、土源性蠕虫感染患者和健康对照之间IFN-γ（P = 0.023）、IL-10（P = 0.008）和TGF-β（P = 0.0001）的细胞因子水平显著不同。恶性疟原虫疟疾、土源性蠕虫感染患者以及恶性疟原虫疟疾和土源性蠕虫共感染患者的IL-10（P = 0.004）、IL-6（P = 0.011）和TGF-β（P = 0.003）的细胞因子水平显著不同。IFN-γ在恶性疟原虫疟疾期间上调，IL-10和TGF-β在土源性蠕虫感染中上调。我们证明恶性疟原虫和土传蠕虫的共感染导致IL-10和IL-6的上调和TGF-β的下调。试验注册编号17/10-16
Malaria and helminths share the same geographical distribution in tropical Africa. Studies of the interaction of helminth and malaria co-infection in humans have been few and are mainly epidemiological, with little information on cellular immune responses. This study aimed to determine Cytokine profiles among patients co-infected with Plasmodium falciparum malaria and soil borne helminth attending Kampala International University Teaching Hospital (KIU). A case control study of 240 patients were recruited at KIU teaching hospital. Patients with Plasmodium falciparum malaria were 55 (22.9%) and those with soil-borne helminths were 63 (26.3%). The controls were 89 (37.1%), while those co-infected with Plasmodium falciparum malaria and soil-borne helminths were 33 (13.8%). Cases were defined as having a positive blood smear for P. falciparum malaria, those with helminths or co-infections of the two. Negative controls were those with a negative blood smear for P. falciparum malaria and those with no stool parasitic infections. Patients presenting with signs and symptoms of malaria or those suspected of having helminths were recruited for the study. A panel of five cytokines (IFN-γ, TNF-α, IL-6, TGF-β and IL-10) were assayed from plasma samples in patients with and without Plasmodium falciparum malaria, patients with and without helminth, and then those co-infected with the two diseases diagnosis was done using thick blood smears stained with 10% Giemsa and stool examination was done following the Kato Katz technique following standard procedures. The prevalence of Plasmodium falciparum malaria by sex was 28 (11.7%) and 27 (11.3%) in male and female respectively. The overall prevalence of soil borne helminth was 26.3%, and among those harbouring helminths, 13.8% were co-infected with Plasmodium falciparum. Cytokine levels significantly differed across Plasmodium falciparum malaria, soil borne helminth infected patients and health controls for IFN-γ (P = 0.023), IL-10 (P = 0.008) and TGF-β (P = 0.0001). Cytokine levels significantly differed across Plasmodium falciparum malaria, soil borne helminth infected patients and patients co-infected with Plasmodium falciparum malaria and soil borne helminth for IL-10 (P = 0.004), IL-6 (P = 0.011) and TGF-β (P = 0.003). An up-regulation of IFN-γ during Plasmodium falciparum malaria and an up-regulation of IL-10 and TGF-β in soil borne helminth infections was demonstrated. We demonstrate that co-infections of Plasmodium falciparum and soil borne helminth lead to an up-regulation of IL-10 and IL-6 and a down-regulation of TGF-β. Trial registration No17/10-16
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DOI：
10.4269/ajtmh.2005.73.1124
发表时间：
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影响因子：
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