Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic.
Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic.
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DOI：
10.1371/journal.pone.0037283
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
de Sá Morais LL
中科院分区：
文献类型：
--
作者：
Garza DR;Thompson CC;Loureiro EC;Dutilh BE;Inada DT;Junior EC;Cardoso JF;Nunes MR;de Lima CP;Silvestre RV;Nunes KN;Santos EC;Edwards RA;Vicente AC;de Sá Morais LL
第7次霍乱大流行于1991年蔓延到拉丁美洲，从秘鲁蔓延到几乎所有拉美国家。在疫情后期，一种未能发酵蔗糖的菌株主导了巴西北部亚马逊地区的霍乱疫情。为了了解这种蔗糖发酵表型改变的基因组特征和决定因素，对菌株IEC224的基因组进行了测序。本文报告了对该毒株的广泛基因组研究，显示了它与主要流行谱系的相关性。潜在的移动基因组区域具有GC含量偏差，并且含有霍乱弧菌的主要毒力基因。应用一种新的生物信息学方法来识别假想蛋白质的可能功能，并与RAST的自动标注进行了比较。一个大型噬菌体的基因组被发现整合到IEC224的S丙氨酸氨基肽酶基因上。这种噬菌体的存在被证明是来自拉丁美洲疫情的El Tor菌株的共同特征，以及它可能来自安哥拉的祖先。缺陷的蔗糖发酵表型被证明是由于霍乱弧菌蔗糖特异性运输基因中的单核苷酸插入所致。这种移码突变截断了一种膜蛋白，改变了它的结构孔状构象。此外，对一种常见噬菌体的鉴定加强了1991年拉丁美洲霍乱流行的主要病原体的单系学和非洲起源假说。
The 7th cholera pandemic reached Latin America in 1991, spreading from Peru to virtually all Latin American countries. During the late epidemic period, a strain that failed to ferment sucrose dominated cholera outbreaks in the Northern Brazilian Amazon region. In order to understand the genomic characteristics and the determinants of this altered sucrose fermenting phenotype, the genome of the strain IEC224 was sequenced. This paper reports a broad genomic study of this strain, showing its correlation with the major epidemic lineage. The potentially mobile genomic regions are shown to possess GC content deviation, and harbor the main V. cholera virulence genes. A novel bioinformatic approach was applied in order to identify the putative functions of hypothetical proteins, and was compared with the automatic annotation by RAST. The genome of a large bacteriophage was found to be integrated to the IEC224's alanine aminopeptidase gene. The presence of this phage is shown to be a common characteristic of the El Tor strains from the Latin American epidemic, as well as its putative ancestor from Angola. The defective sucrose fermenting phenotype is shown to be due to a single nucleotide insertion in the V. cholerae sucrose-specific transportation gene. This frame-shift mutation truncated a membrane protein, altering its structural pore-like conformation. Further, the identification of a common bacteriophage reinforces both the monophyletic and African-Origin hypotheses for the main causative agent of the 1991 Latin America cholera epidemics.
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