Development of a simplified spinal cord ischemia model in mice.
Development of a simplified spinal cord ischemia model in mice.
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DOI：
10.1016/j.jneumeth.2010.04.003
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发表时间：
2010-06-15
影响因子：
3
通讯作者：
Sheng, Huaxin
中科院分区：
文献类型：
--
作者：
Wang, Zhengfeng;Yang, Wei;Britz, Gavin W.;Lombard, Frederick W.;Warner, David S.;Sheng, Huaxin
使用基因改造小鼠有助于了解许多疾病的病理机制，并有助于治疗的发展。然而，目前还没有可用于脊髓缺血的实用且临床相关的小鼠模型。本报告介绍了一种简化的脊髓缺血长期结果小鼠模型。雄性C57Bl/6J小鼠用异氟烷麻醉并气管内插管。通过左侧开胸术夹闭胸主动脉中段0、8、10或12分钟。直肠温度维持在37.0±0.5℃。激光多普勒探头用于测量胸主动脉交叉夹紧期间腰部脊髓血流量。在受伤后 1 小时以及 1、3、5 和 7 天评估旷场运动功能和旋转杆性能。损伤后 7 天对腰椎腹角中存活的神经元进行计数。交叉夹闭胸主动脉中段导致腰脊髓血流量减少约 90%。神经功能缺损和神经元细胞死亡与缺血持续时间相关。另一组小鼠接受10分钟的主动脉夹闭或假手术，并在1小时和1、3、5、7、14和28天检查神经功能。受伤组的 5 只小鼠中有 4 只 (80%) 存活了 28 天，并且有明显的神经功能缺损。这项研究表明，通过左胸廓切开术交叉夹紧主动脉是诱导小鼠脊髓缺血的一种简单而可靠的方法，可以确定长期结果。
Use of genetically manipulated mice facilitates understanding pathological mechanisms in many diseases and contributes to therapy development. However, there is no practical and clinically relevant mouse model available for spinal cord ischemia. This report introduces a simplified long-term outcome mouse model of spinal cord ischemia. Male C57Bl/6J mice were anesthetized with isoflurane and endotracheally intubated. The middle segment of the thoracic aorta was clamped for 0, 8, 10 or 12min via left lateral thoracotomy. Rectal temperature was maintained at 37.0±0.5°C. A laser Doppler probe was used to measure lumbar spinal cord blood flow during thoracic aorta cross-clamping. Open field locomotor function and rotarod performance were evaluated at 1h and 1, 3, 5, and 7 days post-injury. Surviving neurons in the lumbar ventral horn were counted at 7 days post-injury. Cross-clamping the middle segment of the thoracic aorta resulted in approximately 90% blood flow reduction in the lumbar spinal cord. Neurological deficit and neuronal cell death were associated with ischemia duration. Another set of mice were subjected to 10min aortic clamping or sham surgery and neurological function was examined at 1h and 1, 3, 5, 7, 14, and 28 days. Four of 5 mice (80%) in the injured group survived 28 days and had significant neurological deficit. This study indicates that cross-clamping of the aorta via left thoracotomy is a simple and reliable method to induce spinal cord ischemia in mice allowing definition of long-term outcome.
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影响因子：
6.3
作者：
MARSALA, M;YAKSH, TL
通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.051626798
发表时间：
2001-03-13
影响因子：
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通讯作者：
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影响因子：
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作者：
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