FAT1 inhibits cell migration and invasion by affecting cellular mechanical properties in esophageal squamous cell carcinoma.
FAT1 inhibits cell migration and invasion by affecting cellular mechanical properties in esophageal squamous cell carcinoma.
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FAT1通过影响食管鳞状细胞癌的细胞力学特性抑制细胞迁移和侵袭
DOI：
10.3892/or.2018.6328
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发表时间：
2018-05
期刊：
影响因子：
4.2
通讯作者：
Cheng X
中科院分区：
文献类型：
--
作者：
Hu X;Zhai Y;Shi R;Qian Y;Cui H;Yang J;Bi Y;Yan T;Yang J;Ma Y;Zhang L;Liu Y;Li G;Zhang M;Cui Y;Kong P;Cheng X
脂肪非典型钙粘蛋白1（FAT1）属于钙粘蛋白超家族，它调节细胞间粘附和其他细胞行为，在人类肿瘤中起着重要作用。我们以前确定FAT 1是食管鳞状细胞癌（ESCC）的重要突变基因之一。在本研究中，我们用慢病毒敲低YSE 2和Colo 680 N细胞系中的FAT 1表达，发现FAT 1的敲低导致细胞迁移和侵袭的加速。通过原子力显微镜（AFM）检测细胞粘附力和细胞弹性力，发现抑制内源性FAT1表达导致细胞粘附力降低，细胞弹性力增加。总之，我们的研究表明，FAT1改变细胞的机械性能，导致失调的细胞迁移和ESCC的侵袭，这可能是一个新的ESCC治疗的目标。
FAT atypical cadherin 1 (FAT1) belongs to the cadherin superfamily and has been reported to regulate cell-cell adhesion and other cell behaviors, suggesting its pivotal roles in human cancers. We previously identified FAT1 as one of the significant mutant genes in esophageal squamous cell carcinoma (ESCC). In the present study, the knockdown of FAT1 expression in YSE2 and Colo680N cell lines was carried out by lentivirus, and we found that knockdown of FAT1 led to acceleration of cell migration and invasion. Furthermore, we detected the cell adhesive force and cell elasticity force by atomic force microscopy (AFM) and found that the suppression of endogenous expression of FAT1 led to a decrease in the cell adhesive force and increase in the cell elasticity force compared with the control groups. In conclusion, our study demonstrated that FAT1 altered cellular mechanical properties leading to deregulation of cell migration and invasion of ESCC, which may be a novel target for ESCC therapy.
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影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
影响因子：
9.2
作者：
Bennett, F. Christian;Harvey, Kieran F.
通讯作者：
Harvey, Kieran F.
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
30.8
作者：
Lin DC;Hao JJ;Nagata Y;Xu L;Shang L;Meng X;Sato Y;Okuno Y;Varela AM;Ding LW;Garg M;Liu LZ;Yang H;Yin D;Shi ZZ;Jiang YY;Gu WY;Gong T;Zhang Y;Xu X;Kalid O;Shacham S;Ogawa S;Wang MR;Koeffler HP
通讯作者：
Koeffler HP
影响因子：
5.2
作者：
Katoh M
通讯作者：
Katoh M