Selenium speciation in urine by ion-pairing chromatography with perfluorinated carboxylic acids and ICP-MS detection
Selenium speciation in urine by ion-pairing chromatography with perfluorinated carboxylic acids and ICP-MS detection
复制标题
通过全氟羧酸离子对色谱法和 ICP-MS 检测对尿液中的硒形态进行分析
DOI：
10.1039/b202256g
复制
发表时间：
2002
影响因子：
3.4
通讯作者：
O. Jøns
中科院分区：
文献类型：
--
作者：
B. Gammelgaard;L. Bendahl;U. Sidenius;O. Jøns
五个水标准，硒代蛋氨酸（SeMet），甲基硒代蛋氨酸（MeSeMet），甲基硒代半胱氨酸（MeSeCys），硒γ-氨基丁酸（SeGaba）和三甲基硒离子（TMS e），分离的离子对色谱系统的基础上全氟羧酸在甲醇中。两种不同的全氟羧酸，七氟丁酸（HFBA）和九氟戊酸（NFPA），被用来作为离子对剂的分离。离子对试剂的选择性不同。在微孔柱上进行分离，所述微孔柱通过实验室制备的直接注射雾化器连接至ICP-MS。当使用50 µl min−1的流速时，该雾化系统允许洗脱液中的甲醇浓度为50%。尿液中的检测限为0.8至1.7 µg l−1，
绝对检测限之间的2.3和5.1 pg。尿液样品从不同的个人之前和期间补充硒蛋氨酸进行了分析。在不同的尿样中分离出几种物质。在色谱图开始时洗脱的主要组分在许多样品中占主导地位，特别是在硒消耗后。该物质未被鉴定，固相萃取实验表明其为中性。当不同的尿液样本加标可用的标准品时，在一些样本中观察到物质与TMS e、MeSeMet或SeMet的共洗脱。这些物种都不是尿液样本中的主要化合物即使在大量摄入含硒补充剂之后。尿样中的硒物种显示出有限的稳定性，因为它们在+4 °C和-18 °C储存期间会发生变化。
Five aqueous standards, selenomethionine (SeMet), methylselenomethionine (MeSeMet), methylselenocysteine (MeSeCys), selenogammaaminobutyric acid (SeGaba) and the trimethylselenonium ion (TMSe), were separated in ion-pairing chromatographic systems based on perfluorinated carboxylic acids in methanol. Two different perfluorinated carboxylic acids, heptafluorobutanoic acid (HFBA) and nonafluoropentanoic acid (NFPA), were used as ion-pairing agents in the separation. The selectivities of the ion-pairing agents were different. The separation was performed on a microbore column, which was connected to the ICP-MS via a laboratory-made direct injection nebuliser. This nebulisation system allowed methanol concentrations of 50% in the eluent when a flow rate of 50 µl min−1 was used. The detection limits in urine were between 0.8 and 1.7 µg l−1 corresponding
to absolute detection limits of between 2.3 and 5.1 pg. Urine samples from different individuals before and during supplementation with selenomethionine were analysed. Several species were separated in the different urine samples. A major component eluting at the beginning of the chromatogram was predominant in many samples, especially after selenium consumption. This species was not identified and solid phase extraction experiments suggested that it was neutral. When different urine samples were spiked with the available standards, co-elution of species with TMSe, MeSeMet or SeMet was observed in some samples. None of these species were major compounds in urine samples—even after massive consumption of selenium-containing supplements. The selenium species in the urine samples showed a limited stability, as they changed during storage at +4 °C as well as −18 °C.
三甲基硒离子在人尿中的排泄。
影响因子：
2.9
作者：
Sun,XF;Ting,BT;Janghorbani,M
通讯作者：
Janghorbani,M
由[75Se]硒代胱氨酸合成[75Se]三甲基碘化硒。
影响因子：
2.9
作者：
Foster,SJ;Ganther,HE
通讯作者：
Ganther,HE