Sewage surveillance of SARS-CoV-2 at student campus residences in the Western Cape, South Africa.

Sewage surveillance of SARS-CoV-2 at student campus residences in the Western Cape, South Africa.
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DOI:
10.1016/j.scitotenv.2022.158028
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发表时间:
2022-12-10
影响因子:
9.8
通讯作者:
Johnson, Rabia
Johnson, Rabia
中科院分区:
环境科学与生态学1区
文献类型:
--
作者:
Mangwana, Noluxabiso;Archer, Edward;Muller, Christo J. F.;Preiser, Wolfgang;Wolfaardt, Gideon;Kasprzyk-Hordern, Barbara;Carstens, Alno;Brocker, Ludwig;Webster, Candice;McCarthy, David;Street, Renee;Mathee, Angela;Louw, Johan;Mdhluli, Mongezi;Johnson, Rabia

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目前严重急性呼吸系统综合征冠状病毒2型(SARS-CoV-2)的诊断能力在特定社区有限,对追踪和追踪新感染构成挑战。通过有针对性的废水监测来监测被认为是感染热点的学生宿舍至关重要。这项研究评估了南非斯泰伦博斯大学学生宿舍SARS-CoV-2针对性废水监测对缓解疫情的有效性。使用鱼雷式被动采样装置,每两周从斯泰伦博斯大学Tygerberg(SUT)校园和斯泰伦博斯大学主(SUM)校园学生宿舍的沙井中收集废水样本。通过监测,对校园内出现的SARS-CoV-2进行了预警检测,随后采取了知情的管理战略,限制了学生在校园内的活动,并推迟了全国各地经历的第三波疫情的爆发。此外,该研究强调了即使报告了少量确诊的2019年冠状病毒病(COVID-19)病例,也可能在指定地点感染的程度。该研究还使用Thermo Fisher TaqMan® RT-qPCR基因分型检测跟踪了学生宿舍中Delta和Omicron变体的激增。
The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnostic capacity is limited in defined communities, posing a challenge in tracking and tracing new infections. Monitoring student residences, which are considered infection hotspots, with targeted wastewater surveillance is crucial. This study evaluated the efficacy of SARS-CoV-2 targeted wastewater surveillance for outbreak mitigation at Stellenbosch University's student residences in South Africa. Using torpedo-style passive sampling devices, wastewater samples were collected biweekly from manholes at twelve Stellenbosch University Tygerberg (SUT) campus and Stellenbosch University-Main (SUM) campus student residences. The surveillance led to an early warning detection of SARS-CoV-2 presence on campus, followed by an informed management strategy leading to restriction of student activities on campus and a delay in the onset of the third wave that was experienced throughout the country. Moreover, the study highlighted the extent of possible infections at defined locations even when a low number of confirmed coronavirus disease 2019 (COVID-19) cases were reported. The study also tracked the surge of the Delta and Omicron variants in the student residences using the Thermo Fisher TaqMan® RT-qPCR genotyping assay.
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