A sensitive, simple, and low-cost method for COVID-19 wastewater surveillance at an institutional level.
A sensitive, simple, and low-cost method for COVID-19 wastewater surveillance at an institutional level.
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一种灵敏、简单、低成本的方法，用于机构层面的COVID-19废水监测。
DOI：
10.1016/j.scitotenv.2021.151047
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发表时间：
2022-02-10
期刊：
影响因子：
--
通讯作者：
Moe CL
中科院分区：
文献类型：
--
作者：
Liu P;Ibaraki M;VanTassell J;Geith K;Cavallo M;Kann R;Guo L;Moe CL
SARS-CoV-2是一种呼吸道病毒，但也在相当大比例的新冠肺炎病例的粪便样本中检测到它。最近的研究表明，与追踪社区新冠肺炎疫情的大规模诊断测试相比，废水监测可能是一种低成本的工具，但大多数研究都集中在污水处理厂的采样上。机构一级的废水监测可以很好地用于预警目的，因为可以跟踪出现新病例的特定地理区域/人口，并在废水信号呈阳性时立即采取行动。在这项研究中，开发了一种新的摩尔拭子方法，并将其用于机构层面的新冠肺炎废水监测。在检测的442个拭子样本中，148个(33.5%)从三个校园和两个大楼采集的拭子对SARS-CoV-2RNA呈阳性。对已知病例数量的隔离大楼的进一步研究表明，该方法足够灵敏，可以在大楼内检测到很少的病例。此外，对医院排污管道的抓取样本和摩尔拭子样本的比较表明，摩尔拭子比抓取样本更敏感，提供了一种简单、廉价的方法，可以在24-48小时内获得废水中的病毒复合样本。这些结果表明，收集和分析摩尔拭子用于检测SARS-CoV-2RNA是一种敏感、低成本和易于使用的新冠肺炎监测工具，适用于机构环境，可以部署在低资源环境中来识别社区中的新兴新冠肺炎集群。
SARS-CoV-2 is a respiratory virus, but it is also detected in a significant proportion of fecal samples from COVID-19 cases. Recent studies have shown that wastewater surveillance can be a low-cost tool compared to massive diagnostic testing for tracking COVID-19 outbreaks in communities, but most studies have focused on sampling from wastewater treatment plants. Institutional level wastewater surveillance may serve well for early warning purposes because specific geographic areas/populations with emerging cases can be tracked and immediate action can be executed in the event of a positive wastewater signal. In this study, a novel Moore swab method was developed and used for wastewater surveillance of COVID-19 at an institutional level. Of the 442 swab samples tested, 148 (33.5%) swabs collected from the three campuses and two buildings were positive for SARS-CoV-2 RNA. Further study of the quarantine building with a known number of cases indicated that this method was sensitive enough to detect few cases in the building. In addition, comparison between grab samples and Moore swab samples from the hospital sewage line indicated that Moore swabs were more sensitive than grab samples and offer a simple, inexpensive method for obtaining a composite sample of virus in wastewater over a 24–48 h period. These results suggest that collection and analyses of Moore swabs for SARS-CoV-2 RNA detection is a sensitive, low-cost, and easy to use tool for COVID-19 surveillance that is useful for institutional settings and could be deployed in low-resource settings to identify emerging COVID-19 clusters in communities.
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影响因子：
5.2
作者：
Liu P;Ibaraki M;Kapoor R;Amin N;Das A;Miah R;Mukhopadhyay AK;Rahman M;Dutta S;Moe CL
通讯作者：
Moe CL
影响因子：
2.7
作者：
Johannessen, Erik;Eikum, Arild Schanke;Krogstad, Tore
通讯作者：
Krogstad, Tore
影响因子：
4.4
作者：
Deshpande, JM;Shetty, SJ;Siddiqui, ZA
通讯作者：
Siddiqui, ZA
DOI：
10.1016/j.coesh.2020.09.006
发表时间：
2020-10
影响因子：
--
作者：
Medema G;Been F;Heijnen L;Petterson S
通讯作者：
Petterson S
同时检测来自加利福尼亚中部海岸农业地区的水样中的人类诺如病毒和细菌病原体。
影响因子：
5.2
作者：
Tian P;Yang D;Shan L;Wang D;Li Q;Gorski L;Lee BG;Quiñones B;Cooley MB
通讯作者：
Cooley MB