Traditional processing increases biological activities of Dendrobium offificinale Kimura et. Migo in Southeast Yunnan, China.
Traditional processing increases biological activities of Dendrobium offificinale Kimura et. Migo in Southeast Yunnan, China.
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DOI：
10.1038/s41598-022-17628-8
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发表时间：
2022-08-31
影响因子：
4.6
通讯作者：
Yang, Lixin
中科院分区：
文献类型：
--
作者：
Zhou, Di;Zhao, Ying;Chen, Zhilin;Yan, Xiuxiang;Zhao, Yanqiang;Gao, Lu;Yang, Lixin
铁皮石斛为兰科植物，遍及东南部、中国和东南亚国家，在传统中药中用于治疗炎症和糖尿病。铁皮风豆是一种著名的中药材，由干燥的铁皮石杉茎制成。炮制改变了TPFD的物理化学性质；然而，加工如何影响这种植物的质量和药用价值尚不清楚。本研究采用定性和定量相结合的方法，分析比较了铁皮石斛鲜茎的化学成分，探讨了其炮制后药效增强的可能原因。为了鉴定FSD和TPFD的成分，我们在负离子和正离子模式下使用了超高效液相色谱结合质谱仪，并使用人类代谢组数据库和多元统计分析对数据进行了解释。我们检测到23,709个峰，鉴定出2352个代谢物；其中370个代谢物在FSD和TPFD之间差异丰富(TPFD比FSD丰富245个，比FSD少125个)，包括有机氧化合物、戊烯醇脂类、类黄酮、羧酸及其衍生物和脂肪酰基。其中，43个化学标记明确区分了FSD和TPFD样品，经正交偏最小二乘判别分析证实。药理活性分析表明，与FSD相比，TPFD具有明显更高的抗炎活性代谢物水平，与其用于治疗炎症的作用一致。除了揭示TPFD药效的基础外，这项研究还支持传统用法的好处。
The orchid Dendrobium officinale grows throughout southeast China and southeast Asian countries and is used to treat inflammation and diabetes in traditional Chinese medicine. Tie pi feng dou is a well-known traditional Chinese medicine made from the dried D. officinale stems. Processing alters the physicochemical properties of TPFD; however, it is unclear how processing affects the quality and medicinal value of this plant. Here, we analyzed and compared the chemical composition of fresh stems of D. officinale and TPFD and explored possible explanations for the enhanced medicinal efficacy of processed D. officinale stems using qualitative and quantitative methods. To identify the components of FSD and TPFD, we used ultra-high-performance liquid chromatography combined with mass spectrometry in negative and positive ion modes and interpreted the data using the Human Metabolome Database and multivariate statistical analysis. We detected 23,709 peaks and identified 2352 metabolites; 370 of these metabolites were differentially abundant between FSD and TPFD (245 more abundant in TPFD than in FSD, and 125 less abundant), including organooxygen compounds, prenol lipids, flavonoids, carboxylic acids and their derivatives, and fatty acyls. Of these, 43 chemical markers clearly distinguished between FSD and TPFD samples, as confirmed using orthogonal partial least squares discriminant analysis. A pharmacological activity analysis showed that, compared with FSD, TPFD had significantly higher levels of some metabolites with anti-inflammatory activity, consistent with its use to treat inflammation. In addition to revealing the basis of the medicinal efficacy of TPFD, this study supports the benefits of the traditional usage of D. officinale.
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影响因子：
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