Distinct hypothalamic control of same- and opposite-sex mounting behaviour in mice.
Distinct hypothalamic control of same- and opposite-sex mounting behaviour in mice.
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DOI：
10.1038/s41586-020-2995-0
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发表时间：
2021-01
期刊：
影响因子：
64.8
通讯作者：
Anderson DJ
中科院分区：
文献类型：
--
作者：
Karigo T;Kennedy A;Yang B;Liu M;Tai D;Wahle IA;Anderson DJ
表面上相似的动物行为可以在不同的环境中表达不同的意图，但这种灵活性是如何在神经回路层面上实现的尚不清楚。例如，许多物种的雄性可以表现出对同性或异性同种的行为，但目前还不清楚这些行为的意图和神经编码是相似还是不同。在这里，我们表明，女性和男性定向安装在雄性实验室小鼠的存在或不存在的超声发声（USVs），分别是可区分的。这些和其他行为数据表明，大多数男性直接安装是侵略性的，虽然在极少数情况下，它可以是性的。我们研究了USV+和USV−安装是否使用相同或不同的下丘脑神经基质。在内侧视前区（MPOA）或腹内侧下丘脑腹外侧亚区（VMHvl）中雌激素受体1（ESR 1）阳性神经元的显微内窥镜成像显示了USV+和USV-安装期间不同的神经元活动模式，安装类型可以从任一区域的群体活动中解码。表达ESR 1和囊泡GABA转运蛋白（VGAT）的MPOA神经元（MPOAESR 1 → VGAT神经元）的交叉光遗传学刺激强烈促进USV+安装，并将雄性定向攻击转化为USV安装。相比之下，刺激表达ESR 1的VMHvl神经元（VMHvlESR 1神经元）促进USV-安装，并抑制女性尿液诱发的USV。终末刺激实验表明，这些互补的抑制作用介导的MPOA和VMHvl之间的相互投射。总之，这些数据确定了一个下丘脑亚群，是遗传富集的神经元，因果诱导男性生殖行为状态，并表明生殖和侵略性的状态是由不同的人口代码分布在MPOAESR 1和VMHvlESR 1神经元，分别代表。因此，表达不同内部状态的类似行为由不同的下丘脑神经元群体编码。
Animal behaviours that are superficially similar can express different intents in different contexts, but how this flexibility is achieved at the level of neural circuits is not understood. For example, males of many species can exhibit mounting behaviour towards same- or opposite-sex conspecifics, but it is unclear whether the intent and neural encoding of these behaviours are similar or different. Here we show that female- and male-directed mounting in male laboratory mice are distinguishable by the presence or absence of ultrasonic vocalizations (USVs), respectively. These and additional behavioural data suggest that most male-directed mounting is aggressive, although in rare cases it can be sexual. We investigated whether USV+ and USV− mounting use the same or distinct hypothalamic neural substrates. Micro-endoscopic imaging of neurons positive for oestrogen receptor 1 (ESR1) in either the medial preoptic area (MPOA) or the ventromedial hypothalamus, ventrolateral subdivision (VMHvl) revealed distinct patterns of neuronal activity during USV+ and USV− mounting, and the type of mounting could be decoded from population activity in either region. Intersectional optogenetic stimulation of MPOA neurons that express ESR1 and vesicular GABA transporter (VGAT) (MPOAESR1∩VGAT neurons) robustly promoted USV+ mounting, and converted male-directed attack to mounting with USVs. By contrast, stimulation of VMHvl neurons that express ESR1 (VMHvlESR1 neurons) promoted USV− mounting, and inhibited the USVs evoked by female urine. Terminal stimulation experiments suggest that these complementary inhibitory effects are mediated by reciprocal projections between the MPOA and VMHvl. Together, these data identify a hypothalamic subpopulation that is genetically enriched for neurons that causally induce a male reproductive behavioural state, and indicate that reproductive and aggressive states are represented by distinct population codes distributed between MPOAESR1 and VMHvlESR1 neurons, respectively. Thus, similar behaviours that express different internal states are encoded by distinct hypothalamic neuronal populations.
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影响因子：
48
作者：
Kim CK;Yang SJ;Pichamoorthy N;Young NP;Kauvar I;Jennings JH;Lerner TN;Berndt A;Lee SY;Ramakrishnan C;Davidson TJ;Inoue M;Bito H;Deisseroth K
通讯作者：
Deisseroth K
下丘脑前区域的分子，空间和功能性单细胞分析。
DOI：
10.1126/science.aau5324
发表时间：
2018-11-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Moffitt JR;Bambah-Mukku D;Eichhorn SW;Vaughn E;Shekhar K;Perez JD;Rubinstein ND;Hao J;Regev A;Dulac C;Zhuang X
通讯作者：
Zhuang X
影响因子：
3.7
作者：
Matsumoto YK;Okanoya K
通讯作者：
Okanoya K
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
25
作者：
Stagkourakis, Stefanos;Spigolon, Giada;Broberger, Christian
通讯作者：
Broberger, Christian