QTL Analysis of Z414, a Chromosome Segment Substitution Line with Short, Wide Grains, and Substitution Mapping of qGL11 in Rice.
QTL Analysis of Z414, a Chromosome Segment Substitution Line with Short, Wide Grains, and Substitution Mapping of qGL11 in Rice.
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水稻短粒宽粒染色体片段替代系Z414的QTL分析及qGL11的替代作图
DOI：
10.1186/s12284-022-00571-7
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发表时间：
2022-05-09
期刊：
影响因子：
5.5
通讯作者：
Zhao, Fangming
中科院分区：
文献类型：
--
作者：
Li, Juan;Yang, Hongxia;Xu, Guangyi;Deng, Keli;Yu, Jinjin;Xiang, Siqian;Zhou, Kai;Zhang, Qiuli;Li, Ruxiang;Li, Miaomiao;Ling, Yinghua;Yang, Zhenglin;He, Guanghua;Zhao, Fangming
关键词：
水稻（Oryza sativa）的大多数农艺性状，如粒长，都是由多个基因控制的复杂性状。染色体片段代换系（CSSLs）是剖析这些复杂性状的理想材料。我们从受体亲本“西恢18”（籼型恢复系）和供体亲本“沪旱3号”（粳稻品种）的后代中培育出了新型水稻CSSL“Z414”，其谷粒短而宽。Z414含有4个代换片段，平均长度为3.04 Mb。Z414表现出7个性状与西恢18显著不同，包括粒长、粒宽和粒重、垩白度以及糙米率的差异。我们在西恢18与Z414杂交所得F2群体中鉴定出7个与这些差异相关的数量性状位点（QTL）。其中，6个QTL（qPL3、qGW5、qGL11、qRLW5、qRLW11和qGWT5）在新培育的单片段代换系（SSSLs）S1 - S6中被检测到。此外，4个QTL（qGL3、qGL5、qCD3和qCD5）在S1和S5中被检测到。对这些SSSLs的分析表明，Z414短而宽的谷粒性状归因于qGL11、qGL3、qGL5和qGW5。代换定位将qGL11界定在第11号染色体上一个810 kb的区间内。测序、实时定量PCR和细胞形态分析表明，qGL11可能是一个编码细胞周期蛋白CycT1;3的新QTL。最后，聚合qGL3（a = 0.43）和qGL11（a = - 0.37）使得双片段代换系D2的谷粒更短，并且表明qGL11对qGL3上位。此外，S1和D2的谷粒大小与Z414不同，且垩白度比Z414低。总之，CSSL Z414的短粒表型受qGL11、qGL3和qGL5控制。qGL11可能是一个编码CycT1;3的新QTL，其在调控粒长方面的具体作用此前未知，且qGL11对qGL3上位。S1和D2有潜力应用于杂交水稻育种。
在线版本包含补充材料，网址为10.1186/s12284 - 022 - 00571 - 7。
Most agronomic traits of rice (Oryza sativa), such as grain length, are complex traits controlled by multiple genes. Chromosome segment substitution lines (CSSLs) are ideal materials for dissecting these complex traits. We developed the novel rice CSSL ‘Z414’, which has short, wide grains, from progeny of the recipient parent ‘Xihui 18’ (an indica restorer line) and the donor parent ‘Huhan 3’ (a japonica cultivar). Z414 contains four substitution segments with an average length of 3.04 Mb. Z414 displays seven traits that significantly differ from those of Xihui 18, including differences in grain length, width, and weight; degree of chalkiness; and brown rice rate. We identified seven quantitative trait loci (QTL) that are responsible for these differences in an F2 population from a cross between Xihui 18 and Z414. Among these, six QTL (qPL3, qGW5, qGL11, qRLW5, qRLW11, and qGWT5) were detected in newly developed single-segment substitution lines (SSSLs) S1–S6. In addition, four QTL (qGL3, qGL5, qCD3, and qCD5) were detected in S1 and S5. Analysis of these SSSLs attributed the short, wide grain trait of Z414 to qGL11, qGL3, qGL5, and qGW5. Substitution mapping delimited qGL11 within an 810-kb interval on chromosome 11. Sequencing, real time quantitative PCR, and cell morphology analysis revealed that qGL11 might be a novel QTL encoding the cyclin CycT1;3. Finally, pyramiding qGL3 (a = 0.43) and qGL11 (a = − 0.37) led to shorter grains in the dual-segment substitution line D2 and revealed that qGL11 is epistatic to qGL3. In addition, S1 and D2 exhibited different grain sizes and less chalkiness than Z414. In conclusion, the short grain phenotype of the CSSL Z414 is controlled by qGL11, qGL3, and qGL5. qGL11 might be a novel QTL encoding CycT1;3, whose specific role in regulating grain length was previously unknown, and qGL11 is epistatic to qGL3. S1 and D2 could potentially be used in hybrid rice breeding. The online version contains supplementary material available at 10.1186/s12284-022-00571-7.
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