Hidden diversity of Ctenophora revealed by new mitochondrial COI primers and sequences.
Hidden diversity of Ctenophora revealed by new mitochondrial COI primers and sequences.
复制标题
DOI：
10.1111/1755-0998.13459
复制
发表时间：
2022-01
影响因子：
7.7
通讯作者：
中科院分区：
文献类型：
--
作者：
线粒体基因细胞色素c氧化酶亚基1 （COI）在许多分类群的系统发育、群体遗传学、元条形码和快速物种鉴定中都很有用。然而，栉水母门（栉水母）由于线粒体序列的差异以及相应的无法用退化的和标准的COI“条形码”引物扩增COI，在历史上一直难以研究。因此，尽管在栉水母门中有超过200种已被描述的物种，但可用于栉水母的COI序列非常少。在这里，我们设计了新的引物，并扩增了所有主要栉水母群体成员的COI片段，包括许多未被描述的物种。对COI序列的系统发育分析显示，在许多类群中具有较高的多样性，这在更保守的18S rDNA序列中并不明显，特别是在Lobata （Ctenophora; Tentaculata; Lobata）中。COI系统发育结果还揭示了Bolinopsis属内意想不到的群落结构，提出了Bathocyroe属内的新种，并支持了一些物种的生态和形态差异，如Lampocteis cruentiventer和类似的未描述的裂片（Lampocteis sp.“V”按深度分层，“A”按颜色区分）。本文报道的新设计引物为研究人员提供了重要的工具，使他们能够通过快速分子鉴定来阐明世界范围内栉水母的多样性，通过改进参考文库和扩增来提高分析环境DNA的能力，并使群体遗传研究的广度得到新的扩展。
The mitochondrial gene cytochrome‐c‐oxidase subunit 1 (COI) is useful in many taxa for phylogenetics, population genetics, metabarcoding, and rapid species identifications. However, the phylum Ctenophora (comb jellies) has historically been difficult to study due to divergent mitochondrial sequences and the corresponding inability to amplify COI with degenerate and standard COI “barcoding” primers. As a result, there are very few COI sequences available for ctenophores, despite over 200 described species in the phylum. Here, we designed new primers and amplified the COI fragment from members of all major groups of ctenophores, including many undescribed species. Phylogenetic analyses of the resulting COI sequences revealed high diversity within many groups that was not evident from more conserved 18S rDNA sequences, in particular among the Lobata (Ctenophora; Tentaculata; Lobata). The COI phylogenetic results also revealed unexpected community structure within the genus Bolinopsis, suggested new species within the genus Bathocyroe, and supported the ecological and morphological differences of some species such as Lampocteis cruentiventer and similar undescribed lobates (Lampocteis sp. “V” stratified by depth, and “A” differentiated by colour). The newly designed primers reported herein provide important tools to enable researchers to illuminate the diversity of ctenophores worldwide via quick molecular identifications, improve the ability to analyse environmental DNA by improving reference libraries and amplifications, and enable a new breadth of population genetic studies.
登录
查看更多内容
影响因子：
2.6
作者：
Lacoursière-Roussel A;Howland K;Normandeau E;Grey EK;Archambault P;Deiner K;Lodge DM;Hernandez C;Leduc N;Bernatchez L
通讯作者：
Bernatchez L
影响因子：
2.8
作者：
Leray M;Yang JY;Meyer CP;Mills SC;Agudelo N;Ranwez V;Boehm JT;Machida RJ
通讯作者：
Machida RJ
DOI：
10.1016/0146-6291(78)90590-8
发表时间：
1978-01-01
期刊：
DEEP-SEA RESEARCH
影响因子：
--
作者：
HARBISON, GR;MADIN, LP;SWANBERG, NR
通讯作者：
SWANBERG, NR
DOI：
10.1017/s0025315400041217
发表时间：
1978-01-01
影响因子：
1.2
作者：
MADIN, LP;HARBISON, GR
通讯作者：
HARBISON, GR
影响因子：
3.4
作者：
Arafat H;Alamaru A;Gissi C;Huchon D
通讯作者：
Huchon D