eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity.
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity.
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DOI：
10.1002/ece3.4213
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发表时间：
2018-08
影响因子：
2.6
通讯作者：
Bernatchez L
中科院分区：
文献类型：
--
作者：
Lacoursière-Roussel A;Howland K;Normandeau E;Grey EK;Archambault P;Deiner K;Lodge DM;Hernandez C;Leduc N;Bernatchez L
由于北极目前正在发生重大的全球变化，因此建立一个大规模的北极海洋生物多样性标准化数据库尤为紧迫。本研究评估了水生环境DNA （eDNA）元条形码检测北极沿海生物多样性变化的潜力，并在两个地点表征了eDNA的局部时空分布。我们利用两对COI引物对从加拿大两个北极港口——丘吉尔港和伊瓜伊特港采集的约80个水样中提取和扩增了eDNA，并基于优化的采样和保存方法进行了偏远地区的调查。结果表明，水生eDNA调查具有记录大规模北极生物多样性变化的潜力，可以提供沿海后生动物生物多样性的快速概述，检测非本地物种，并允许在开放水域和冰盖下由当地北部社区采样。我们发现大约50%的已知加拿大北极物种和潜在入侵者的DNA序列目前存在于公共数据库中。eDNA元条形码在物种水平上鉴定出相似比例的可操作分类单元，在两个地点共鉴定出181个物种。尽管沿海环境寒冷且混合良好，但物种组成在垂直方向上是不均匀的，部分原因是河口生态系统的河流流入，并且在水柱和潮池之间存在差异。因此，基于COI的eDNA元条形码可以快速改善使用eDNA的大规模北极生物监测，但我们警告说，水生eDNA采样需要在空间和时间上标准化，以准确评估群落结构变化。
Because significant global changes are currently underway in the Arctic, creating a large‐scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) metabarcoding to detect Arctic coastal biodiversity changes and characterizes the local spatio‐temporal distribution of eDNA in two locations. We extracted and amplified eDNA using two COI primer pairs from ~80 water samples that were collected across two Canadian Arctic ports, Churchill and Iqaluit, based on optimized sampling and preservation methods for remote regions surveys. Results demonstrate that aquatic eDNA surveys have the potential to document large‐scale Arctic biodiversity change by providing a rapid overview of coastal metazoan biodiversity, detecting nonindigenous species, and allowing sampling in both open water and under the ice cover by local northern‐based communities. We show that DNA sequences of ~50% of known Canadian Arctic species and potential invaders are currently present in public databases. A similar proportion of operational taxonomic units was identified at the species level with eDNA metabarcoding, for a total of 181 species identified at both sites. Despite the cold and well‐mixed coastal environment, species composition was vertically heterogeneous, in part due to river inflow in the estuarine ecosystem, and differed between the water column and tide pools. Thus, COI‐based eDNA metabarcoding may quickly improve large‐scale Arctic biomonitoring using eDNA, but we caution that aquatic eDNA sampling needs to be standardized over space and time to accurately evaluate community structure changes.
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影响因子：
4.6
作者：
Eguíluz VM;Fernández-Gracia J;Irigoien X;Duarte CM
通讯作者：
Duarte CM
调查环境 DNA (eDNA) 在海洋哺乳动物遗传监测中的潜在用途。
影响因子：
3.7
作者：
Foote AD;Thomsen PF;Sveegaard S;Wahlberg M;Kielgast J;Kyhn LA;Salling AB;Galatius A;Orlando L;Gilbert MT
通讯作者：
Gilbert MT
DOI：
10.1139/cjfas-2014-0473
发表时间：
2015-08-01
影响因子：
2.4
作者：
Chan, Farrah T.;MacIsaac, Hugh J.;Bailey, Sarah A.
通讯作者：
Bailey, Sarah A.
影响因子：
3.7
作者：
Elbrecht V;Leese F
通讯作者：
Leese F
影响因子：
7.7
作者：
Geller, J.;Meyer, C.;Hawk, H.
通讯作者：
Hawk, H.