Air-Filled Bubbles Stabilized by Gold Nanoparticle/Photodynamic Dye Hybrid Structures for Theranostics.
Air-Filled Bubbles Stabilized by Gold Nanoparticle/Photodynamic Dye Hybrid Structures for Theranostics.
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DOI：
10.3390/nano11020415
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发表时间：
2021-02-06
期刊：
影响因子：
--
通讯作者：
Gorin DA
中科院分区：
文献类型：
--
作者：
Barmin RA;Rudakovskaya PG;Gusliakova OI;Sindeeva OA;Prikhozhdenko ES;Maksimova EA;Obukhova EN;Chernyshev VS;Khlebtsov BN;Solovev AA;Sukhorukov GB;Gorin DA
微泡已经作为血管造影的超声造影剂进入临床实践。然而，需要对气泡的外壳进行修改，以生产用于超声波和多模态（荧光/光声）成像方法并结合治疗诊断（诊断和治疗）的探针。在目前的工作中，基于具有空气核和壳的微泡的混合结构由牛血清白蛋白、白蛋白包被的金纳米颗粒和临床可用的光动力染料（锌酞菁、吲哚菁绿）组成，可实现多模态成像，在光动力治疗中具有潜在的应用。获得并表征了平均尺寸为 1.5 ± 0.3 μm、浓度高达 1.2 × 109 微泡/mL的微泡。将染料引入系统降低了溶液的表面张力，从而提高了气泡的浓度和稳定性。描述了金纳米粒子和光动力染料的组合对荧光信号和探针稳定性的影响。使用荧光断层扫描、光栅扫描光声显微镜和使用频率为 33 MHz 的医用超声设备进行超声响应测量来评估所获得的探针在生物医学应用中的潜在用途。结果证明了使用金纳米颗粒/光动力染料混合结构实现微泡稳定性的影响，以实现治疗诊断学中的探针应用。
Microbubbles have already reached clinical practice as ultrasound contrast agents for angiography. However, modification of the bubbles’ shell is needed to produce probes for ultrasound and multimodal (fluorescence/photoacoustic) imaging methods in combination with theranostics (diagnostics and therapeutics). In the present work, hybrid structures based on microbubbles with an air core and a shell composed of bovine serum albumin, albumin-coated gold nanoparticles, and clinically available photodynamic dyes (zinc phthalocyanine, indocyanine green) were shown to achieve multimodal imaging for potential applications in photodynamic therapy. Microbubbles with an average size of 1.5 ± 0.3 μm and concentration up to 1.2 × 109 microbubbles/mL were obtained and characterized. The introduction of the dye into the system reduced the solution’s surface tension, leading to an increase in the concentration and stability of bubbles. The combination of gold nanoparticles and photodynamic dyes’ influence on the fluorescent signal and probes’ stability is described. The potential use of the obtained probes in biomedical applications was evaluated using fluorescence tomography, raster-scanning optoacoustic microscopy and ultrasound response measurements using a medical ultrasound device at the frequency of 33 MHz. The results demonstrate the impact of microbubbles’ stabilization using gold nanoparticle/photodynamic dye hybrid structures to achieve probe applications in theranostics.
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用于光声成像的金纳米粒子
DOI：
10.2217/nnm.14.169
发表时间：
2015-01
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
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DOI：
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发表时间：
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影响因子：
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