A catalog of reference genomes from the human microbiome.
A catalog of reference genomes from the human microbiome.
复制标题
DOI：
10.1126/science.1183605
复制
发表时间：
2010-05-21
期刊：
影响因子：
--
通讯作者：
Zhu D
中科院分区：
文献类型：
--
作者：
Human Microbiome Jumpstart Reference Strains Consortium;Nelson KE;Weinstock GM;Highlander SK;Worley KC;Creasy HH;Wortman JR;Rusch DB;Mitreva M;Sodergren E;Chinwalla AT;Feldgarden M;Gevers D;Haas BJ;Madupu R;Ward DV;Birren BW;Gibbs RA;Methe B;Petrosino JF;Strausberg RL;Sutton GG;White OR;Wilson RK;Durkin S;Giglio MG;Gujja S;Howarth C;Kodira CD;Kyrpides N;Mehta T;Muzny DM;Pearson M;Pepin K;Pati A;Qin X;Yandava C;Zeng Q;Zhang L;Berlin AM;Chen L;Hepburn TA;Johnson J;McCorrison J;Miller J;Minx P;Nusbaum C;Russ C;Sykes SM;Tomlinson CM;Young S;Warren WC;Badger J;Crabtree J;Markowitz VM;Orvis J;Cree A;Ferriera S;Fulton LL;Fulton RS;Gillis M;Hemphill LD;Joshi V;Kovar C;Torralba M;Wetterstrand KA;Abouellleil A;Wollam AM;Buhay CJ;Ding Y;Dugan S;FitzGerald MG;Holder M;Hostetler J;Clifton SW;Allen-Vercoe E;Earl AM;Farmer CN;Liolios K;Surette MG;Xu Q;Pohl C;Wilczek-Boney K;Zhu D
人类的微生物组是指填充人体的微生物，包括原核生物，病毒和微生物的真核生物。包括数百个微生物参考基因组的测序，与来自多个身体部位的宏基因组测序结合。从178个微生物基因组的初始参考基因组测序中。 NR子集被定义。该分析产生了一组30,867个多肽，其中29,987（〜97％）是唯一的一组微生物基因组允许基于使用的匹配标准与泛基因组分析相关的胃肠道的微生物组40％。此外，提出了该组用于质量保证的相关指标和标准。
The human microbiome refers to the community of microorganisms including prokaryotes, viruses and microbial eukaryotes that populate the human body. The National Institutes of Health launched an initiative that focuses describing the diversity of microbial species associated with health and disease. The first phase of this initiative includes the sequencing of hundreds of microbial reference genomes, coupled to metagenomic sequencing from multiple body sites. Here we present results from an initial reference genome sequencing of 178 microbial genomes. From 547,968 predicted polypeptides that correspond to the gene complement of these strains “novel” polypeptides that had both unmasked sequence length > 100 amino acids and no BLASTP match to any non-reference entry in the nr subset were defined. This analysis resulted in a set of 30,867 polypeptides, of which 29,987 (~97%) were unique. In addition, this set of microbial genomes allows for ~ 40% of random sequences from the microbiome of the gastrointestinal tract to be associated with organisms based on the match criteria used. Insights into pan-genome analysis suggest that we are still far from saturating microbial species genetic datasets. In addition, the associated metrics and standards used by the group for quality assurance are presented.
登录
查看更多内容
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4.4
作者：
Konstantinidis, Konstantinos T.;Ramette, Alban;Tiedje, James M.
通讯作者：
Tiedje, James M.
DOI：
10.1016/0097-8485(93)85006-x
发表时间：
1993-06-01
期刊：
COMPUTERS & CHEMISTRY
影响因子：
--
作者：
WOOTTON, JC;FEDERHEN, S
通讯作者：
FEDERHEN, S
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
9.8
作者：
Rusch DB;Halpern AL;Sutton G;Heidelberg KB;Williamson S;Yooseph S;Wu D;Eisen JA;Hoffman JM;Remington K;Beeson K;Tran B;Smith H;Baden-Tillson H;Stewart C;Thorpe J;Freeman J;Andrews-Pfannkoch C;Venter JE;Li K;Kravitz S;Heidelberg JF;Utterback T;Rogers YH;Falcón LI;Souza V;Bonilla-Rosso G;Eguiarte LE;Karl DM;Sathyendranath S;Platt T;Bermingham E;Gallardo V;Tamayo-Castillo G;Ferrari MR;Strausberg RL;Nealson K;Friedman R;Frazier M;Venter JC
通讯作者：
Venter JC