Genotyping of Anopheles mosquito blood meals reveals nonrandom human host selection: implications for human-to-mosquito Plasmodium falciparum transmission.
Genotyping of Anopheles mosquito blood meals reveals nonrandom human host selection: implications for human-to-mosquito Plasmodium falciparum transmission.
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DOI：
10.1186/s12936-023-04541-2
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发表时间：
2023-04-07
期刊：
影响因子：
3
通讯作者：
Cohee, Lauren M. M.
中科院分区：
文献类型：
--
作者：
Mbewe, Rex B. B.;Keven, John B. B.;Mangani, Charles;Wilson, Mark L. L.;Mzilahowa, Themba;Mathanga, Don P. P.;Valim, Clarissa;Laufer, Miriam K. K.;Walker, Edward D. D.;Cohee, Lauren M. M.
关键词：
Control of malaria parasite transmission can be enhanced by understanding which human demographic groups serve as the infectious reservoirs. Because vector biting can be heterogeneous, some infected individuals may contribute more to human-to-mosquito transmission than others. Infection prevalence peaks in school-age children, but it is not known how often they are fed upon. Genotypic profiling of human blood permits identification of individual humans who were bitten. The present investigation used this method to estimate which human demographic groups were most responsible for transmitting malaria parasites to Anopheles mosquitoes. It was hypothesized that school-age children contribute more than other demographic groups to human-to-mosquito malaria transmission. In a region of moderate-to-high malaria incidence in southeastern Malawi, randomly selected households were surveyed to collect human demographic information and blood samples. Blood-fed, female Anopheles mosquitoes were sampled indoors from the same houses. Genomic DNA from human blood samples and mosquito blood meals of human origin was genotyped using 24 microsatellite loci. The resultant genotypes were matched to identify which individual humans were sources of blood meals. In addition, Plasmodium falciparum DNA in mosquito abdomens was detected with polymerase chain reaction. The combined results were used to identify which humans were most frequently bitten, and the P. falciparum infection prevalence in mosquitoes that resulted from these blood meals. Anopheles females selected human hosts non-randomly and fed on more than one human in 9% of the blood meals. Few humans contributed most of the blood meals to the Anopheles vector population. Children ≤ 5 years old were under-represented in mosquito blood meals while older males (31–75 years old) were over-represented. However, the largest number of malaria-infected blood meals was from school age children (6–15 years old). The results support the hypothesis that humans aged 6–15 years are the most important demographic group contributing to the transmission of P. falciparum to the Anopheles mosquito vectors. This conclusion suggests that malaria control and prevention programmes should enhance efforts targeting school-age children and males. The online version contains supplementary material available at 10.1186/s12936-023-04541-2.
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影响因子：
4.6
作者：
Jeyaprakasam NK;Low VL;Liew JWK;Pramasivan S;Wan-Sulaiman WY;Saeung A;Vythilingam I
通讯作者：
Vythilingam I
影响因子：
9.8
作者：
通讯作者：
--
DOI：
10.1016/s1473-3099(21)00072-4
发表时间：
2021-11
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Andolina C;Rek JC;Briggs J;Okoth J;Musiime A;Ramjith J;Teyssier N;Conrad M;Nankabirwa JI;Lanke K;Rodriguez-Barraquer I;Meerstein-Kessel L;Arinaitwe E;Olwoch P;Rosenthal PJ;Kamya MR;Dorsey G;Greenhouse B;Drakeley C;Staedke SG;Bousema T
通讯作者：
Bousema T
DOI：
10.1073/pnas.1405617111
发表时间：
2014-07-29
影响因子：
11.1
作者：
De Moraes, Consuelo M.;Stanczyk, Nina M.;Mescher, Mark C.
通讯作者：
Mescher, Mark C.
影响因子：
3
作者：
Coalson JE;Cohee LM;Buchwald AG;Nyambalo A;Kubale J;Seydel KB;Mathanga D;Taylor TE;Laufer MK;Wilson ML
通讯作者：
Wilson ML