Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells.
Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells.
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DOI：
10.3892/mmr.2017.7568
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发表时间：
2017-11
影响因子：
3.4
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Ji W;Liu S;Zhao X;Guo Y;Xia S;Lu Y;Yin M;Xu X
强直性脊柱炎(AS)的特征是骨刺的形成。治疗由此产生的强直、过度的骨形成和相关的功能损害，仍然是关于这种情况的研究的主要治疗目标。雷公藤甲素是多年生藤本植物雷公藤的主要活性成分。已经证明具有抗肿瘤活性，包括抑制细胞生长和诱导细胞凋亡，但雷公藤甲素对成骨细胞的作用尚不清楚。本研究将不同浓度的雷公藤甲素作用于小鼠成骨细胞系MC3T3-E1不同的时间间隔。用溴脱氧尿嘧啶核苷比色法检测细胞增殖，用流式细胞仪检测细胞周期和凋亡率，用Hoechst染色观察细胞核凋亡，用Western印迹分析检测相关蛋白。用含雷公藤甲素的成骨诱导液培养7~12d后，用天狼星染色、碱性磷酸酶活性观察和钙沉积实验检测细胞向成骨细胞分化的情况。结果表明，雷公藤甲素可显著抑制成骨细胞的增殖，诱导成骨细胞周期停滞和细胞凋亡。此外，雷公藤甲素处理减少了胶原形成、碱性磷酸酶活性和钙沉积。本研究证实了雷公藤甲素对成骨细胞增殖和分化的抑制作用，为将来治疗AS提供了一种潜在的治疗药物。
Ankylosing spondylitis (AS) is characterized by the formation of bony spurs. Treatment of the resulting ankylosis, excessive bone formation and associated functional impairment, remain the primary therapeutic aims in research regarding this condition. Triptolide is the primary active component of the perennial vine Tripterygium wilfordii Hook. f., and has previously been demonstrated to exert anti-tumor activities including inhibition of cell growth and the induction of apoptosis, however, the effect of triptolide on osteoblasts remains to be elucidated. In the present study, the MC3T3-E1 mouse osteoblast cell line was treated with differing concentrations of triptolide for various intervals. Cell proliferation was detected using the bromodeoxyuridine assay, cell cycle and apoptosis were measured by flow cytometry, nuclear apoptosis was observed by Hoechst staining and associated proteins were determined via western blot analysis. The cells were then further incubated with osteogenic induction medium supplemented with triptolide for 7 or 12 days and the differentiation to osteoblasts was examined by picrosirius staining, observation of alkaline phosphatase activity and a calcium deposition assay. It was demonstrated that treatment with triptolide significantly inhibited osteoblast proliferation and induced cell cycle arrest and apoptosis of the osteoblasts. Furthermore, treatment with triptolide reduced collagen formation, alkaline phosphatase activity and calcium deposition. The present study demonstrated an inhibitory effect of triptolide on osteoblast proliferation and differentiation, and therefore suggests a potential therapeutic agent for the treatment of AS in the future.
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影响因子：
5.6
作者：
OWEN, TA;ARONOW, M;STEIN, GS
通讯作者：
STEIN, GS
雷公藤内酯醇诱导人肾细胞癌细胞的细胞周期停滞和细胞凋亡。
影响因子：
4.2
作者：
Li, Jingjie;Zhu, Wenbo;Yan, Guangmei
通讯作者：
Yan, Guangmei
DOI：
10.1002/ar.22479
发表时间：
2012-06-01
影响因子：
2
作者：
Wang, Jixi;Wang, Aibing;Xu, Yudong
通讯作者：
Xu, Yudong
雷公藤中的两种主要化合物雷公藤甲素和雷公藤红素的协同抗癌作用
影响因子：
--
作者：
Jiang QW;Cheng KJ;Mei XL;Qiu JG;Zhang WJ;Xue YQ;Qin WM;Yang Y;Zheng DW;Chen Y;Wei MN;Zhang X;Lv M;Chen MW;Wei X;Shi Z
通讯作者：
Shi Z
影响因子：
3
作者：
Qiu, Daoming;Kao, Peter N
通讯作者：
Kao, Peter N