Muscularis macrophages establish cell-to-cell contacts with telocytes/PDGFRα-positive cells and smooth muscle cells in the human and mouse gastrointestinal tract.
Muscularis macrophages establish cell-to-cell contacts with telocytes/PDGFRα-positive cells and smooth muscle cells in the human and mouse gastrointestinal tract.
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肌层巨噬细胞与人和小鼠胃肠道中的特细胞/PDGFRα阳性细胞和平滑肌细胞建立细胞间接触
DOI：
10.1111/nmo.13993
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发表时间：
2021-03
影响因子：
3.5
通讯作者：
Cipriani G
中科院分区：
文献类型：
--
作者：
Ji S;Traini C;Mischopoulou M;Gibbons SJ;Ligresti G;Faussone-Pellegrini MS;Sha L;Farrugia G;Vannucchi MG;Cipriani G
肌层巨噬细胞（Muscularis macrophages，MMG）不仅是先天免疫的介质，而且在功能上与胃肠运动的重要细胞相互作用。本研究的目的是确定人和小鼠的胃、小肠和大肠的固有肌层中的细胞与邻近细胞之间的空间关系和接触类型。通过免疫组织化学和透射电子显微镜观察MM的分布、形态及其与其他细胞的接触。免疫组化显示，根据其在肌被不同部位的位置，不同形状和数量的肌上皮细胞。通过双标记，观察到了神经元和邻近细胞之间的密切联系，即神经元、平滑肌细胞、Cajal间质细胞（ICC）、端细胞（TC）/PDGFRα阳性细胞。电子显微镜检查表明，在两种动物种属的肌肉层中，平滑肌细胞和TC/PDGFRα阳性细胞具有相似的超微结构特征，并且与平滑肌细胞和TC/PDGFRα阳性细胞有专门的细胞间接触，但与ICC和肠神经元无接触。这项研究描述了肠道不同区域之间的不同分布模式，并报告了肠道平滑肌细胞和平滑肌细胞之间以及肠道平滑肌细胞和TC/PDGFRα阳性细胞之间存在不同和扩展的细胞间接触。相比之下，虽然接近ICC和神经元，但它并不与ICC接触。这些研究结果表明，专门和变量的作用，为胃肠道动力的调制，其意义应在正常和病理条件下进行更密切的调查。
Muscularis macrophages (MMs) are not only mediators of innate immunity, but, also, functionally interact with cells important for gastrointestinal motility. The aim of this study is to determine the spatial relationship and types of contacts between the MMs and neighbouring cells in the muscularis propria of human and mouse stomach, small and large intestine. The distribution and morphology of MMs and their contacts with other cells were investigated by immunohistochemistry and transmission electron microscopy. Immunohistochemistry showed variable shape and number of MMs according to their location in different portions of the muscle coat. By double labeling, a close association between MMs and neighbouring cells, i.e. neurons, smooth muscle cells, interstitial cells of Cajal (ICCs), telocytes (TCs)/PDGFRα-positive cells, was seen. Electron microscopy demonstrated that in the muscle layers of both animal species, MMs have similar ultrastructural features and have specialised cell-to-cell contacts with smooth muscle cells and TCs/PDGFRα-positive cells but not with ICCs and enteric neurons. This study describes varying patterns of distribution of MMs between different regions of the gut, and reports the presence of distinct and extended cell-to-cell contacts between MMs and smooth muscle cells and between MMs and TCs/PDGFRα-positive cells. In contrast, MMs, although close to ICCs and nerve elements did not make contact with them. These findings indicate specialized and variable roles for MMs in the modulation of gastrointestinal motility whose significance should be more closely investigated in normal and pathological conditions.
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影响因子：
5.3
作者：
Chen X;Zheng Y;Manole CG;Wang X;Wang Q
通讯作者：
Wang Q
影响因子：
5.5
作者：
Kurahashi, Masaaki;Zheng, Haifeng;Sanders, Kenton M.
通讯作者：
Sanders, Kenton M.
影响因子：
3.6
作者：
MIKKELSEN, HB;RUMESSEN, JJ
通讯作者：
RUMESSEN, JJ
影响因子：
29.4
作者：
Grover M;Farrugia G;Lurken MS;Bernard CE;Faussone-Pellegrini MS;Smyrk TC;Parkman HP;Abell TL;Snape WJ;Hasler WL;Ünalp-Arida A;Nguyen L;Koch KL;Calles J;Lee L;Tonascia J;Hamilton FA;Pasricha PJ;NIDDK Gastroparesis Clinical Research Consortium
通讯作者：
NIDDK Gastroparesis Clinical Research Consortium
影响因子：
4.6
作者：
Kondo, Ayano;Kaestner, Klaus H.
通讯作者：
Kaestner, Klaus H.