Direct cell-cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo.
Direct cell-cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo.
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成熟成骨细胞和破骨细胞之间的直接细胞 - 细胞接触动态控制其在体内的功能。
DOI：
10.1038/s41467-017-02541-w
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发表时间：
2018-01-19
影响因子：
16.6
通讯作者：
Ishii M
中科院分区：
文献类型：
--
作者：
Furuya M;Kikuta J;Fujimori S;Seno S;Maeda H;Shirazaki M;Uenaka M;Mizuno H;Iwamoto Y;Morimoto A;Hashimoto K;Ito T;Isogai Y;Kashii M;Kaito T;Ohba S;Chung UI;Lichtler AC;Kikuchi K;Matsuda H;Yoshikawa H;Ishii M
骨稳态是由骨形成成熟成骨细胞（mOB）和骨吸收成熟破骨细胞（mOC）之间的通讯调节的。然而，在体内的时空关系和相互作用的模式仍然难以捉摸。在这里，我们表明，通过使用活体成像技术，通过这些细胞类型之间的直接细胞-细胞接触，调节mOB和mOC功能。在稳定状态下，mOB和mOC主要占据离散的区域，尽管在时空有限的区域中检测到直接的细胞与细胞接触。此外，pH敏感荧光探针显示，当它们不与mOBs接触时，mOCs分泌用于骨吸收的质子，而与mOBs接触的mOCs是非吸收性的，这表明mOBs可以通过直接接触抑制骨吸收。甲状旁腺激素的间歇给药引起骨合成代谢作用，这导致mOB和mOC的混合分布，并增加细胞-细胞接触。这项研究揭示了mOBs和mOCs之间的时空细胞间相互作用影响体内骨稳态。成骨细胞和破骨细胞之间的通讯对于骨稳态是必不可少的，但相互作用的模式尚不清楚。作者在小鼠体内使用活体双光子显微镜来显示这些细胞直接相互作用，调节破骨细胞的活性，并且这种相互作用受到甲状旁腺激素给药的调节。
Bone homeostasis is regulated by communication between bone-forming mature osteoblasts (mOBs) and bone-resorptive mature osteoclasts (mOCs). However, the spatial–temporal relationship and mode of interaction in vivo remain elusive. Here we show, by using an intravital imaging technique, that mOB and mOC functions are regulated via direct cell–cell contact between these cell types. The mOBs and mOCs mainly occupy discrete territories in the steady state, although direct cell–cell contact is detected in spatiotemporally limited areas. In addition, a pH-sensing fluorescence probe reveals that mOCs secrete protons for bone resorption when they are not in contact with mOBs, whereas mOCs contacting mOBs are non-resorptive, suggesting that mOBs can inhibit bone resorption by direct contact. Intermittent administration of parathyroid hormone causes bone anabolic effects, which lead to a mixed distribution of mOBs and mOCs, and increase cell–cell contact. This study reveals spatiotemporal intercellular interactions between mOBs and mOCs affecting bone homeostasis in vivo. Communication between osteoblasts and osteoclasts is essential for bone homeostasis, but the mode of interaction is unclear. The authors use intravital two-photon microscopy in mice to show that these cells directly interact, regulating activity of osteoclasts, and that the interaction is modulated by parathyroid hormone administration.
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DOI：
10.1007/978-1-62703-989-5_11
发表时间：
2014-01-01
期刊：
SKELETAL DEVELOPMENT AND REPAIR: METHODS AND PROTOCOLS
影响因子：
--
作者：
Kawamoto, Tadafumi;Kawamoto, Komei
通讯作者：
Kawamoto, Komei
DOI：
10.1084/jem.20101474
发表时间：
2010-12-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ishii M;Kikuta J;Shimazu Y;Meier-Schellersheim M;Germain RN
通讯作者：
Germain RN
影响因子：
4.1
作者：
Bellido T;Saini V;Pajevic PD
通讯作者：
Pajevic PD
影响因子：
82.9
作者：
Nakashima, Tomoki;Hayashi, Mikihito;Takayanagi, Hiroshi
通讯作者：
Takayanagi, Hiroshi
影响因子：
7.8
作者：
PAVLIN, D;LICHTLER, AC;ROWE, DW
通讯作者：
ROWE, DW