An upward 9.4 T static magnetic field inhibits DNA synthesis and increases ROS-P53 to suppress lung cancer growth.
An upward 9.4 T static magnetic field inhibits DNA synthesis and increases ROS-P53 to suppress lung cancer growth.
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向上 9.4 T 静磁场抑制 DNA 合成并增加 ROS-P53 以抑制肺癌生长
DOI：
10.1016/j.tranon.2021.101103
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发表时间：
2021-07
影响因子：
5
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Yang X;Song C;Zhang L;Wang J;Yu X;Yu B;Zablotskii V;Zhang X
9.4 T静磁场向上暴露88小时显著抑制小鼠体内A549肿瘤生长。
9.4 T静磁场处理88小时对小鼠关键器官或血细胞计数无严重损害。
9.4 T静磁场向上处理24小时导致A549 DNA合成受抑制。
9.4 T静磁场向上处理24小时显著提高活性氧（ROS）和P53水平，并导致G2细胞周期阻滞。
研究表明，9.4特斯拉（9.4 T）高场磁共振成像（MRI）在提高图像分辨率和成像能力方面具有明显优势，但在临床批准之前其安全性问题需要进一步验证。同时，新出现的实验证据表明，中高强度静磁场（SMF）具有一些抗癌作用。
我们研究了两种相反静磁场方向对荷肺癌小鼠的影响，发现当荷肺癌小鼠接受9.4 T静磁场总共处理88小时时，9.4 T静磁场向上显著抑制A549肿瘤生长（肿瘤生长抑制率 = 41%），但9.4 T静磁场向下无此作用。体外细胞分析表明，9.4 T静磁场向上处理24小时不仅抑制A549 DNA合成，还显著提高ROS和P53水平，并使G2细胞周期阻滞。此外，9.4 T静磁场处理88小时对小鼠关键器官或血细胞计数无严重损害。
我们的研究结果证明了9.4 T静磁场长期暴露在其未来MRI应用中的安全性，并揭示了9.4 T静磁场向上方向的抗癌潜力。
Upward 9.4 T SMF exposure for 88 h significantly inhibited A549 tumor growth in mice. 9.4 T SMF treatment for 88 h had no severe impairment to the mice key organs or blood cell count. Upward 9.4 T SMF treatment for 24 h caused A549 DNA synthesis inhibition. Upward 9.4 T SMF treatment for 24 h significantly increased ROS and P53 levels, and caused G2 cell cycle arrest. Studies have shown that 9.4 Tesla (9.4 T) high-field magnetic resonance imaging (MRI) has obvious advantages in improving image resolution and capacity, but their safety issues need to be further validated before their clinical approval. Meanwhile, emerging experimental evidences show that moderate to high intensity Static Magnetic Fields (SMFs) have some anti-cancer effects. We examined the effects of two opposite SMF directions on lung cancer bearing mice and found when the lung cancer cell-bearing mice were treated with 9.4 T SMFs for 88 h in total, the upward 9.4 T SMF significantly inhibited A549 tumor growth (tumor growth inhibition=41%), but not the downward 9.4 T SMF. In vitro cellular analysis shows that 9.4 T upward SMF treatment for 24 h not only inhibited A549 DNA synthesis, but also significantly increased ROS and P53 levels, and arrested G2 cell cycle. Moreover, the 9.4 T SMF-treatments for 88 h had no severe impairment to the key organs or blood cell count of the mice. Our findings demonstrated the safety of 9.4 T SMF long-term exposure for their future applications in MRI, and revealed the anti-cancer potential of the upward direction 9.4 T SMF.
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影响因子：
112.7
作者：
Pommier, Yves;Sung, Yilun;Huang, Shar-yin N.;Nitiss, John L.
通讯作者：
Nitiss, John L.
影响因子：
28.2
作者：
Aarts, Marieke;Sharpe, Rachel;Turner, Nicholas C.
通讯作者：
Turner, Nicholas C.
影响因子：
5.7
作者：
Atkinson, Ian C.;Thulborn, Keith R.
通讯作者：
Thulborn, Keith R.
影响因子：
4.6
作者：
Jin, Yuan-Hu;Joo, Hyunjeong;Lee, Choogon
通讯作者：
Lee, Choogon
影响因子：
4.1
作者：
Jagelska, Eva B.;Brazda, Vaclav;Fojta, Miroslav
通讯作者：
Fojta, Miroslav