Different Activity Patterns in Retinal Ganglion Cells of TRPM1 and mGluR6 Knockout Mice.
Different Activity Patterns in Retinal Ganglion Cells of TRPM1 and mGluR6 Knockout Mice.
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DOI：
10.1155/2018/2963232
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发表时间：
2018
影响因子：
--
通讯作者：
Koike C
中科院分区：
文献类型：
--
作者：
Takeuchi H;Horie S;Moritoh S;Matsushima H;Hori T;Kimori Y;Kitano K;Tsubo Y;Tachibana M;Koike C
TRPM1是黑色素瘤相关瞬时受体潜能(TRPM)亚家族的第一个成员，是视网膜双极细胞(BCS)上代谢性谷氨酸受体6(MGluR6)下游的视觉转导通道。人类TRPM1突变与先天性静止性夜盲(CSNB)相关。在TRPM1和mGluR6 KO小鼠视网膜中，Off而不是On对光刺激有反应。在这里，我们报告了TRPM1基因敲除(KO)和mGluR6 KO小鼠视网膜之间的意外差异。我们使用多电极阵列(MEA)记录视网膜神经节细胞(RGC)的尖峰信号。我们在TRPM1KO视网膜中发现自发振荡，而在mGluR6KO视网膜中未发现自发振荡。我们对BCS上的视杆突触终末进行了结构分析。有趣的是，TRPM1KO视网膜BC终末上的视杆显著小于mGluR6KO视网膜。这些数据表明，双极细胞视杆中TRPM1的缺失，而不是mGluR6的缺失，可能影响突触终末的成熟。我们推测，杆状BCS和AII无长突细胞(ACS)之间的信号受损导致自发振荡。TRPM1和mGluR6都是从光感受器到BC树突的信号通路中的重要组成部分，但它们对BC终末和突触后回路的作用不同。
TRPM1, the first member of the melanoma-related transient receptor potential (TRPM) subfamily, is the visual transduction channel downstream of metabotropic glutamate receptor 6 (mGluR6) on retinal ON bipolar cells (BCs). Human TRPM1 mutations are associated with congenital stationary night blindness (CSNB). In both TRPM1 and mGluR6 KO mouse retinas, OFF but not ON BCs respond to light stimulation. Here we report an unexpected difference between TRPM1 knockout (KO) and mGluR6 KO mouse retinas. We used a multielectrode array (MEA) to record spiking in retinal ganglion cells (RGCs). We found spontaneous oscillations in TRPM1 KO retinas, but not in mGluR6 KO retinas. We performed a structural analysis on the synaptic terminals of rod ON BCs. Intriguingly, rod ON BC terminals were significantly smaller in TRPM1 KO retinas than in mGluR6 KO retinas. These data suggest that a deficiency of TRPM1, but not of mGluR6, in rod ON bipolar cells may affect synaptic terminal maturation. We speculate that impaired signaling between rod BCs and AII amacrine cells (ACs) leads to spontaneous oscillations. TRPM1 and mGluR6 are both essential components in the signaling pathway from photoreceptors to ON BC dendrites, yet they differ in their effects on the BC terminal and postsynaptic circuitry.
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影响因子：
5.7
作者：
Ackman JB;Crair MC
通讯作者：
Crair MC
DOI：
10.1016/j.cub.2016.12.033
发表时间：
2017-02-20
期刊：
Current biology : CB
影响因子：
--
作者：
Mani A;Schwartz GW
通讯作者：
Schwartz GW
影响因子：
3.7
作者：
Biswas S;Haselier C;Mataruga A;Thumann G;Walter P;Müller F
通讯作者：
Müller F
影响因子：
2.5
作者：
Gargini, Claudia;Terzibasi, Eva;Strettoi, Enrica
通讯作者：
Strettoi, Enrica
影响因子：
5.5
作者：
Trenholm, Stuart;Borowska, Joanna;Awatramani, Gautam B.
通讯作者：
Awatramani, Gautam B.