Comparative transcriptomic profiling of peripheral efferent and afferent nerve fibres at different developmental stages in mice.
Comparative transcriptomic profiling of peripheral efferent and afferent nerve fibres at different developmental stages in mice.
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小鼠不同发育阶段外周传出和传入神经纤维的比较转录组分析
DOI：
10.1038/s41598-018-30463-0
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发表时间：
2018-08-10
影响因子：
4.6
通讯作者：
Tang X
中科院分区：
文献类型：
--
作者：
Wang H;Zhou Y;Cong M;Zhang L;Gu X;Tang X
周围神经损伤会损害人类的运动和感觉功能，其功能恢复在很大程度上取决于适当目标的精确神经支配所需的轴突生长。为了更好地了解运动和感觉神经纤维如何选择其末端通路，进行了无偏 cDNA 微阵列分析，以检查小鼠不同发育阶段的外周传出和传入纤维的差异基因表达模式。基因本体论（GO）和京都基因和基因组富集（KEGG）分析揭示了运动和感觉神经纤维发育过程中差异表达基因富集的共同和独特特征。 Ingenuity Pathway Analysis (IPA) 进一步表明，关键的差异表达基因与跨突触神经素-神经肽信号成分和多种γ-氨基丁酸 (GABA) 受体相关。本研究的目的是生成运动和感觉神经元分化/成熟过程中调节的基因网络框架。这些数据可能提供关于决定神经元在周围神经损伤后再生的内在能力的潜在细胞和分子机制的新线索。因此，我们的发现可能有助于进一步开发潜在的干预措施，以提高临床周围神经修复的治疗效率。
Peripheral nerve injury impairs motor and sensory function in humans, and its functional recovery largely depends on the axonal outgrowth required for the accurate reinnervation of appropriate targets. To better understand how motor and sensory nerve fibres select their terminal pathways, an unbiased cDNA microarray analysis was conducted to examine differential gene expression patterns in peripheral efferent and afferent fibres at different developmental stages in mice. Gene ontology (GO) and Kyoto Enrichment of Genes and Genomes (KEGG) analyses revealed common and distinct features of enrichment for differentially expressed genes during motor and sensory nerve fibre development. Ingenuity Pathway Analysis (IPA) further indicated that the key differentially expressed genes were associated with trans-synaptic neurexin-neuroligin signalling components and a variety of gamma-aminobutyric acid (GABA) receptors. The aim of this study was to generate a framework of gene networks regulated during motor and sensory neuron differentiation/maturation. These data may provide new clues regarding the underlying cellular and molecular mechanisms that determine the intrinsic capacity of neurons to regenerate after peripheral nerve injury. Our findings may thus facilitate further development of a potential intervention to manipulate the therapeutic efficiency of peripheral nerve repair in the clinic.
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Slit和Robo家族成员在成年小鼠脊髓和周围神经系统中的表达模式
影响因子：
3.7
作者：
Carr L;Parkinson DB;Dun XP
通讯作者：
Dun XP
DOI：
10.1073/pnas.1003287107
发表时间：
2010-06-22
影响因子：
11.1
作者：
Harvey, Pamela;Gong, BangJian;Frank, Eric
通讯作者：
Frank, Eric
DOI：
10.1002/neu.480210104
发表时间：
1990-01-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
FRANK, E;MENDELSON, B
通讯作者：
MENDELSON, B
影响因子：
16.2
作者：
Fuccillo MV;Földy C;Gökce Ö;Rothwell PE;Sun GL;Malenka RC;Südhof TC
通讯作者：
Südhof TC
影响因子：
5.3
作者：
Gaiarsa JL;Porcher C
通讯作者：
Porcher C