Transcriptional dynamics of endodermal organ formation.
Transcriptional dynamics of endodermal organ formation.
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DOI：
10.1002/dvdy.21810
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发表时间：
2009-01
影响因子：
2.5
通讯作者：
Melton, Douglas A.
中科院分区：
文献类型：
--
作者：
Sherwood, Richard I.;Chen, Tzong-Yang Albert;Melton, Douglas A.
虽然包括肝、胰腺和肠在内的内胚层器官具有重要的治疗意义，但在胚胎发生期间内胚层被分成器官域的机制还没有很好地理解。为了更好地理解这一过程，对早期内胚层器官结构域进行了全局基因表达谱分析。在这项全球分析之后，对关键转录因子进行了动态免疫荧光分析，发现了新的表达模式以及细胞表面蛋白，从而可以前瞻性地分离特定的内胚器官结构域。此外，Cdx 2和Sox 2之间的抑制性相互作用被发现发生在预期的胃-肠边界处，在该边界处形成肝脏和胰腺区域，并且Hlxb 9被揭示为沿背腹轴沿着具有分级表达。这些结果有助于了解内胚层器官发生的机制，并应有助于努力复制这一过程中使用多能干细胞。
Although endodermal organs including the liver, pancreas, and intestine are of significant therapeutic interest, the mechanism by which the endoderm is divided into organ domains during embryogenesis is not well understood. To better understand this process, global gene expression profiling was performed on early endodermal organ domains. This global analysis was followed up by dynamic immunofluorescence analysis of key transcription factors, uncovering novel expression patterns as well as cell surface proteins that allow prospective isolation of specific endodermal organ domains. Additionally, a repressive interaction between Cdx2 and Sox2 was found to occur at the prospective stomach-intestine border, with the hepatic and pancreatic domains forming at this boundary, and Hlxb9 was revealed to have graded expression along the dorsal-ventral axis. These results contribute to understanding the mechanism of endodermal organogenesis and should assist efforts to replicate this process using pluripotent stem cells.
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影响因子：
56.9
作者：
Lammert, E;Cleaver, O;Melton, D
通讯作者：
Melton, D
影响因子：
2.7
作者：
Landry, C;Clotman, F;Rousseau, GG
通讯作者：
Rousseau, GG
影响因子：
64.8
作者：
JONSSON, J;CARLSSON, L;EDLUND, H
通讯作者：
EDLUND, H
影响因子：
2.5
作者：
Kimura, Wataru;Yasugi, Sadao;Fukuda, Kimiko
通讯作者：
Fukuda, Kimiko
影响因子：
30.8
作者：
Harrison, KA;Thaler, J;Kehrl, JH
通讯作者：
Kehrl, JH