Immunoreactivity for Ano1 detects depletion of Kit-positive interstitial cells of Cajal in patients with slow transit constipation.
Immunoreactivity for Ano1 detects depletion of Kit-positive interstitial cells of Cajal in patients with slow transit constipation.
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DOI：
10.1111/j.1365-2982.2011.01729.x
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发表时间：
2011-08
影响因子：
3.5
通讯作者：
Farrugia G
中科院分区：
文献类型：
--
作者：
Kashyap P;Gomez-Pinilla PJ;Pozo MJ;Cima RR;Dozois EJ;Larson DW;Ordog T;Gibbons SJ;Farrugia G
Cajal间质细胞（ICC）的耗竭与几种胃肠动力障碍有关。ICC网络的变化通常通过受体酪氨酸激酶试剂盒的免疫标记来检测。Ano 1（DOG 1或TMEM 16 A）最近被描述为胃肠道ICC的标志物。我们的目的是确定Ano 1-免疫反应性是否可以作为一个可靠的标记物，从运动障碍患者的组织ICC。研究了来自具有正常ICC数量的患者的四种组织和来自具有慢传输型便秘和Kit阳性ICC丧失的患者的四种组织。用抗Kit和Ano 1的抗血清进行双标记检测ICC。对照组和慢传输型便秘组织中ICC的突起和胞体均为Ano 1免疫反应阳性。Kit和Ano 1免疫反应性之间几乎完全重叠。慢传输型便秘患者的组织中含有的Ano 1阳性ICC显著少于对照组织。通过Ano 1和Kit免疫反应性鉴定的ICC数量在ICC数量范围内几乎相同，平均每个视野1.64至7.05个细胞，R2值为0.99。Ano 1是检测人类ICC网络变化的可靠和敏感的标志物。用Ano 1选择性抗体标记可重复检测慢传输型便秘患者组织中Kit阳性ICC的耗竭。
Depletion of interstitial cells of Cajal (ICC) is associated with several gastrointestinal motility disorders. Changes in ICC networks are usually detected by immunolabeling for the receptor tyrosine kinase Kit. Ano1 (DOG1 or TMEM16A) was recently described as a marker of ICC in gastrointestinal tract. Our aim was to determine whether Ano1-immunoreactivity can be used as a reliable marker for ICC in tissues from patients with motility disorders. Four tissues from patients with normal ICC numbers and four tissues from patients with slow transit constipation and loss of Kit-positive ICC were studied. ICC were detected by double labeling using antisera to Kit and Ano1. Both the processes and cell bodies of ICC in tissue from controls and slow transit constipation were immunoreactive for Ano1. There was near complete overlap between Kit and Ano1 immunoreactivity. Tissues from patients with slow transit constipation contained significantly fewer Ano1-positive ICC than control tissues. The numbers of ICC identified by Ano1 and Kit-immunoreactivity were nearly identical across the range of ICC numbers from an average of 1.64 to 7.05 cells per field and correlated with an R2 value of 0.99. Ano1 is a reliable and sensitive marker for detecting changes in ICC networks in humans. Labeling with antibodies selective for Ano1 reproducibly detects depletion of Kit-positive ICC in tissues from patients with slow transit constipation.
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