Staphylococcus aureus immunodominant surface antigen B is a cell-surface associated nucleic acid binding protein.
Staphylococcus aureus immunodominant surface antigen B is a cell-surface associated nucleic acid binding protein.
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DOI：
10.1186/1471-2180-9-61
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发表时间：
2009-03-26
期刊：
影响因子：
4.2
通讯作者：
Jefferson KK
中科院分区：
文献类型：
--
作者：
Mackey-Lawrence NM;Potter DE;Cerca N;Jefferson KK
Staphylococcus aureus immunodominant surface antigen B (IsaB) elicits an immune response during septicemia and is generally classified as a virulence factor, but its biological function remains completely undefined. In an attempt to identify staphylococcal RNA-binding proteins, we designed an RNA Affinity Chromatography assay and subsequently isolated IsaB. Western analysis indicated that IsaB was both secreted and cell-surface associated. Gel Shift analysis confirmed the RNA binding activity but revealed that IsaB bound to any nucleic acid without sequence specificity. IsaB exhibited the highest affinity for double-stranded DNA followed by single-stranded DNA and RNA. Because extracellular DNA has been shown to play a role in biofilm formation, we investigated the biofilm-forming capacity of an isogenic isaB deletion mutant but we found that IsaB did not contribute to biofilm formation under any conditions tested. IsaB is an extracellular nucleic acid binding protein, with little to no sequence specificity, but its role in virulence remains unclear.
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影响因子：
3.2
作者：
Cerca, Nuno;Brooks, Jamie L.;Jefferson, Kimberly K.
通讯作者：
Jefferson, Kimberly K.
影响因子：
3.1
作者：
Cramton, SE;Gerke, C;Götz, F
通讯作者：
Götz, F
影响因子：
5.1
作者：
Barken, Kim B.;Pamp, Suenje J.;Tolker-Nielsen, Tim
通讯作者：
Tolker-Nielsen, Tim
影响因子：
3.2
作者：
Dobinsky, S;Kiel, K;Mack, D
通讯作者：
Mack, D
影响因子：
4.4
作者：
Voyich, JA;Braughton, KR;DeLeo, FR
通讯作者：
DeLeo, FR