The role of potassium and host calcium signaling in Toxoplasma gondii egress.
The role of potassium and host calcium signaling in Toxoplasma gondii egress.
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钾和宿主钙信号在弓形虫排出中的作用。
DOI：
10.1016/j.ceca.2020.102337
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发表时间：
2021-03
期刊：
影响因子：
4
通讯作者：
Moreno SNJ
中科院分区：
文献类型：
--
作者：
Vella SA;Moore CA;Li ZH;Hortua Triana MA;Potapenko E;Moreno SNJ
弓形虫是一种专性细胞内寄生虫，在宿主细胞内的寄生虫空泡（PV）内复制。PV（PVM）的膜含有允许宿主胞质溶胶和PV腔之间的离子和小分子平衡的孔。Ca ~（2+）信号是普遍的，T.弓形虫及其哺乳动物宿主细胞利用Ca 2+信号刺激多种细胞功能。T的出口从宿主细胞中分离弓形虫是弓形虫感染周期的重要步骤。弓形虫和胞质Ca 2+增加启动Ca 2+信号级联反应，其在运动和排出的刺激中达到高潮。在这项工作中，我们证明了细胞内T。弓形虫速殖子能够在宿主信号事件期间从宿主细胞质中摄取Ca 2+。细胞内和细胞外Ca 2+来源在达到成功排出所需的胞质Ca 2+阈值方面都是重要的。在单个寄生虫中观察到两个Ca 2+峰，第二个峰是由Ca 2+进入引起的。我们修补了感染的宿主细胞，以允许递送精确浓度的Ca 2+，用于刺激运动和外出。使用这种修补受感染的宿主细胞的方法，可以确定将宿主胞质Ca 2+增加到特定浓度可以触发外出，这通过降低钾（K+）的浓度而进一步加速。
Toxoplasma gondii is an obligate intracellular parasite and replicates inside a parasitophorous vacuole (PV) within the host cell. The membrane of the PV (PVM) contains pores that permits for equilibration of ions and small molecules between the host cytosol and the PV lumen. Ca2+ signaling is universal and both T. gondii and its mammalian host cell utilize Ca2+ signals to stimulate diverse cellular functions. Egress of T. gondii from host cells is an essential step for the infection cycle of T. gondii and a cytosolic Ca2+ increase initiates a Ca2+ signaling cascade that culminates in the stimulation of motility and egress. In this work, we demonstrate that intracellular T. gondii tachyzoites are able to take up Ca2+ from the host cytoplasm during host signaling events. Both intracellular and extracellular Ca2+ sources are important in reaching a threshold of cytosolic Ca2+ needed for successful egress. Two peaks of Ca2+ were observed in single parasites that egressed with the second peak resulting from Ca2+ entry. We patched infected host cells to allow the delivery of precise concentrations of Ca2+ for stimulation of motility and egress. Using this approach of patching infected host cells allowed to determine that increasing the host cytosolic Ca2+ to a specific concentration can trigger egress, which is further accelerated by diminishing the concentration of potassium (K+).
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DOI：
10.1083/jcb.200309097
发表时间：
2004-06-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Arrizabalaga G;Ruiz F;Moreno S;Boothroyd JC
通讯作者：
Boothroyd JC
弓形虫的裂解周期：15年后。
影响因子：
10.5
作者：
Blader IJ;Coleman BI;Chen CT;Gubbels MJ
通讯作者：
Gubbels MJ
影响因子：
6.7
作者：
Collins CR;Hackett F;Strath M;Penzo M;Withers-Martinez C;Baker DA;Blackman MJ
通讯作者：
Blackman MJ
影响因子：
11.4
作者：
Lourido, Sebastian;Tang, Keliang;Sibley, L. David
通讯作者：
Sibley, L. David
影响因子：
7.2
作者：
Bootman, Martin D.;Bultynck, Geert
通讯作者：
Bultynck, Geert