Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters.
Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters.
复制标题
塞尔维亚多瑙河中的SARS-COV-2 RNA检测与未经处理的废水的排放有关。
DOI：
10.1016/j.scitotenv.2021.146967
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发表时间：
2021-08-20
影响因子：
9.8
通讯作者：
Paunovic, Momir
中科院分区：
文献类型：
--
作者：
Kolarevic, Stoimir;Micsinai, Adrienn;Szanto-Egesz, Reka;Lukacs, Alena;Kracun-Kolarevic, Margareta;Lundy, Lian;Kirschner, Alexander K. T.;Farnleitner, Andreas H.;Djukic, Aleksandar;Colic, Jasna;Nenin, Tanja;Sunjog, Karolina;Paunovic, Momir
在塞尔维亚，收集到的城市废水中只有不到 13% 在排放到环境中之前经过处理。这包括贝尔格莱德（塞尔维亚首都；人口 1,700,000）的所有城市废水排放。先前的研究已经确定了贝尔格莱德原废水排放对多瑙河的影响，本研究调查了此类排放是否也为 SARS-CoV-2 RNA 物质提供了途径。样本是在塞尔维亚 COVID-19 疫情迄今为止最严重的情况下收集的。 2020 年 12 月，在第三波高峰期（就报告病例而言），在贝尔格莱德接收废水负荷的地点采集了抓取样本和复合样本。还对在贝尔格莱德上游和下游采集的样本进行了分析。使用 RT-qPCR 和针对核衣壳（N1 和 N2）和包膜 (E) 蛋白基因的引物组对 RNA 进行定量。仅在受废水严重影响的地点收集的样本中检测到 SARS-CoV-2 RNA（5.97 × 103 至 1.32 × 104 拷贝/L），所有三种应用的引物组均发出阳性信号。测定的浓度与其他国家处理厂采样的废水流入物中报告的浓度相对应，表明废水处理不良的国家中污染负荷高的河流所用方法的流行病学指标函数。
In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.
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废水处理厂中职业暴露于SARS-COV-2的定量微生物风险评估（QMRA）。
DOI：
10.1016/j.scitotenv.2020.142989
发表时间：
2021-04-01
期刊：
The Science of the total environment
影响因子：
--
作者：
Dada AC;Gyawali P
通讯作者：
Gyawali P
影响因子：
9.8
作者：
Guerrero-Latorre, Laura;Ballesteros, Isabel;Rios-Touma, Blanca
通讯作者：
Rios-Touma, Blanca
影响因子：
9.8
作者：
Ahmed, Warish;Bertsch, Paul M.;Kitajima, Masaaki
通讯作者：
Kitajima, Masaaki
影响因子：
9.8
作者：
Adelodun, Bashir;Ajibade, Fidelis Odedishemi;Choi, Kyung-Sook
通讯作者：
Choi, Kyung-Sook
影响因子：
10.9
作者：
Medema, Gertjan;Heijnen, Leo;Brouwer, Anke
通讯作者：
Brouwer, Anke