Review of recent advances in analytical techniques for the determination of neurotransmitters.
Review of recent advances in analytical techniques for the determination of neurotransmitters.
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DOI：
10.1016/j.aca.2009.08.038
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发表时间：
2009-10-19
影响因子：
6.2
通讯作者：
Kennedy, Robert T.
中科院分区：
文献类型：
--
作者：
Perry, Maura;Li, Qiang;Kennedy, Robert T.
本文综述了近五年来神经递质在体监测和组织分析的方法和进展。该综述主要按神经递质类型组织。可以通过与分析方法耦合的体内采样或植入传感器来监测发射器和相关化合物。采样主要使用微透析进行，但低流量推拉灌注可以提供空间分辨率的优势，同时最大限度地减少与较高流速相关的组织破坏。与这些采样方法相结合的分析技术包括液相色谱法、毛细管电泳法、酶测定法、传感器和质谱法。氨基酸、单胺、神经肽、乙酰胆碱、核苷和可溶性气体神经递质的检测方法已经发展和改进。这些方法在速度和灵敏度方面的进步使时间分辨率得以提高，并增加了可检测化合物的数量。类似的进展已经使得能够改进组织样本的检测，其中主要强调单细胞和其他小样本。传感器为体内监测提供出色的时间和空间分辨率。在应用于儿茶酚胺、吲哚胺和氨基酸方面取得了显著进展。传感器的稳定性、灵敏度和选择性的提高一直是人们最关心的问题。
Methods and advances for monitoring neurotransmitters in vivo or for tissue analysis of neurotransmitters over the last five years are reviewed. The review is organized primarily by neurotransmitter type. Transmitter and related compounds may be monitored by either in vivo sampling coupled to analytical methods or implanted sensors. Sampling is primarily performed using microdialysis, but low-flow push-pull perfusion may offer advantages of spatial resolution while minimizing the tissue disruption associated with higher flow rates. Analytical techniques coupled to these sampling methods include liquid chromatography, capillary electrophoresis, enzyme assays, sensors, and mass spectrometry. Methods for the detection of amino acid, monoamine, neuropeptide, acetylcholine, nucleoside, and soluable gas neurotransmitters have been developed and improved upon. Advances in the speed and sensitivity of these methods have enabled improvements in temporal resolution and increased the number of compounds detectable. Similar advances have enabled improved detection at tissue samples, with a substantial emphasis on single cell and other small samples. Sensors provide excellent temporal and spatial resolution for in vivo monitoring. Advances in application to catecholamines, indoleamines, and amino acids have been prominent. Improvements in stability, sensitivity, and selectivity of the sensors have been of paramount interest.
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影响因子：
2.3
作者：
Baseski, HM;Watson, CJ;Kennedy, RT
通讯作者：
Kennedy, RT
影响因子：
3.3
作者：
Bosch, OJ;Krömer, SA;Neumann, ID
通讯作者：
Neumann, ID
影响因子：
5.7
作者：
Cao, Liwei;Zhang, Huashan;Hong, Wang
通讯作者：
Hong, Wang
DOI：
10.1016/j.jpba.2004.09.029
发表时间：
2005-01-04
影响因子：
3.4
作者：
Cannazza, G;Di Stefano, A;Parenti, C
通讯作者：
Parenti, C
DOI：
10.1016/j.jchromb.2006.08.029
发表时间：
2007-02-01
影响因子：
3
作者：
Berna, Michael J.;Ackermann, Bradley L.
通讯作者：
Ackermann, Bradley L.