Characterization of the lipoxygenase (LOX) gene family in the Chinese white pear (Pyrus bretschneideri) and comparison with other members of the Rosaceae.
Characterization of the lipoxygenase (LOX) gene family in the Chinese white pear (Pyrus bretschneideri) and comparison with other members of the Rosaceae.
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中国白梨 (Pyrus bretschneideri) 脂氧合酶 (LOX) 基因家族的特征及其与蔷薇科其他成员的比较
DOI：
10.1186/1471-2164-15-444
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发表时间：
2014-06-07
期刊：
影响因子：
4.4
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Li M;Li L;Dunwell JM;Qiao X;Liu X;Zhang S
脂氧化酶（lipoxygenases, LOXs）是植物中普遍存在的一种非血红素含铁双加氧酶，参与果实香气的形成，是果实品质的一个重要方面。在各种香气挥发物中，饱和和不饱和醇和醛提供了水果的特有香气。这些化合物是由不饱和脂肪酸经过氧化、热解和还原步骤形成的。这种生物合成途径涉及至少四种酶，包括负责脂质氧化的酶LOX。虽然对拟南芥、大豆、黄瓜和苹果等几种植物的LOX基因家族进行了一些研究，但没有来自梨的信息；而这个基因家族在蔷薇科的进化史仍未确定。结果从5个蔷花科物种（梨、家蝇、花栗、梅和桃李）中鉴定出107个LOX同源基因；其中23个序列来自梨。通过结构分析、系统发育分析和共线性分析，确定了该基因家族的基因结构变异，揭示了该基因家族的系统发育进化关系。通过转录组数据分析，证实了某些梨LOX基因在果实发育过程中的表达。结论在梨中鉴定出23个LOX基因，这些基因发生了30-45 MYA的重复；这23个基因中的大多数都是功能性的。在梨基因组的4号染色体上发现了特定的基因重复。为进一步研究LOX基因的进化历史和转基因研究提供了有益的信息。
BackgroundLipoxygenases (LOXs), a type of non-haem iron-containing dioxygenase, are ubiquitous enzymes in plants and participate in the formation of fruit aroma which is a very important aspect of fruit quality. Amongst the various aroma volatiles, saturated and unsaturated alcohols and aldehydes provide the characteristic aroma of the fruit. These compounds are formed from unsaturated fatty acids through oxidation, pyrolysis and reduction steps. This biosynthetic pathway involves at least four enzymes, including LOX, the enzyme responsible for lipid oxidation. Although some studies have been conducted on the LOX gene family in several species including Arabidopsis, soybean, cucumber and apple, there is no information from pear; and the evolutionary history of this gene family in the Rosaceae is still not resolved.ResultsIn this study we identified 107 LOX homologous genes from five Rosaceous species (Pyrus bretschneideri, Malus× domestica, Fragaria vesca, Prunus mume and Prunus persica); 23 of these sequences were from pear. By using structure analysis, phylogenic analysis and collinearity analysis, we identified variation in gene structure and revealed the phylogenetic evolutionary relationship of this gene family. Expression of certain pear LOX genes during fruit development was verified by analysis of transcriptome data.Conclusions23 LOX genes were identified in pear and these genes were found to have undergone a duplication 30–45 MYA; most of these 23 genes are functional. Specific gene duplication was found on chromosome4 in the pear genome. Useful information was provided for future research on the evolutionary history and transgenic research on LOX genes.
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